Ocean acidification does not limit squid metabolism via blood oxygen supply.

Ocean acidification is hypothesized to limit the performance of squids due to their exceptional oxygen demand and pH-sensitivity of blood-oxygen binding, which may reduce oxygen supply in acidified waters. The critical oxygen partial pressure ( Pcrit ), the P O 2 below which oxygen supply cannot match basal demand, is a commonly reported index of hypoxia tolerance. Any CO 2 -induced reduction in oxygen supply should be apparent as an increase in Pcrit In this study, we assessed the effects of CO 2 (46-143 Pa; 455-1410 μatm ) on the metabolic rate and Pcrit of two squid species - Dosidicus gigas and Doryteuthis pealeii - through manipulative experiments. We also developed a model, with inputs for hemocyanin pH-sensitivity, blood P CO 2 , and buffering capacity that simulates blood oxygen supply under varying seawater CO 2 partial pressures. We compare model outputs to measured Pcrit in squids. Using blood- O 2 parameters from the literature for model inputs, we estimated that, in the absence of blood acid-base regulation, an increase in seawater P CO 2 to 100 Pa (≈ 1000 μatm ) would result in a maximum drop in arterial hemocyanin- O 2 saturation by 1.6% at normoxia and a Pcrit increase of ≈0.5 kPa. Our live-animal experiments support this supposition, as CO 2 had no effect on measured metabolic rate or Pcrit in either squid species.