RP-UPLC method development and validation for the simultaneous estimation of ibuprofen and famotidine in pharmaceutical dosage form.

AIM AND BACKGROUND: A stability-indicating LC method was developed for the simultaneous determination of Ibuprofen and Famotidine in pharmaceutical dosage forms.

MATERIALS AND METHODS: The chromatographic separation was achieved on Acquity UPLC BEH C-18,50 mm × 2.1 mm and 1.7 μm column with gradient elution. The mobile phase A contains a mixture of 50 mM sodium acetate buffer (pH 5.5): methanol (85:15, v/v), and the mobile phase B contains a mixture of 50 mM sodium acetate buffer (pH 5.5): methanol (25:75, v/v). The flow rate was 0.3 mL min(-1), and the detection wavelength was 260 nm.

RESULTS: The limit of detection for Ibuprofen and Famotidine was 1.6 and 1.2 μg mL(-1), respectively. The limit of quantification (LOQ) for Ibuprofen and Famotidine was 5.1 and 4.3 μg mL(-1), respectively.

CONCLUSION: This method was validated for accuracy, precision, and linearity. The method was also found to be stability indicating.