JOURNAL ARTICLE

[Correlations between miRNAs and TGF-β1 in tumor microenvironment of esophageal squamous cell cancer]

Longping Zhu, Liyan Jin, Riyue Jiang, Qianqian Wang, Jian Jiang, Chaoming Mao, Deyu Chen
Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology 2013, 29 (5): 524-8
23643275

OBJECTIVE: To detect the levels of mir-18a-5p, mir-23a-3p, mir-24-3p, mir-25-3p and TGF-β1 in tumor microenvironment of esophageal squamous cell cancer (ESCC) patients and explore their clinical significances and correlations.

METHODS: The mRNA expressions of mir-18a-5p, mir-23a-3p, mir-24-3p, mir-25-3p and TGF-β1 were measured by real-time quantitative RT-PCR in ESCC cell lines ECA-109 and TE-1, normal esophageal squamous epithelial cells, tumor tissues and tumor-adjacent normal tissues from 52 ESCC patients. The expression of TGF-β1 protein in the three types of cells, tumor tissues and tumor-adjacent normal tissues from 52 ESCC patients was detected by Western blotting.

RESULTS: The expressions of mir-18a-5p, mir-24-3p, mir-25-3p and mir-23a-3p in ECA-109 were significantly higher than those in normal esophageal squamous epithelial cells (P<0.05); that is also true of the first three miRNAs in TE-1 (P<0.05). Compared with the normal esophageal squamous epithelial cells, the expression of TGF-β1 was reduced in ECA-109 and TE-1 (P<0.05). Compared with the normal tissues, ESCC tumor tissues were characterized by significant overexpressions of mir-18a-5p, mir-23a-3p, mir-24-3p and mir-25-3p with the rates being 86.5% (45/52), 63.5% (33/52), 78.8% (41/52), 86.5% (45/52), respectively (all P<0.05), and by significantly decreased expression of TGF-β1 (P<0.05). The up-regulation of mir-18a-5p, mir-23a-3p, mir-24-3p, mir-25-3p and the down-regulation of TGF-β1 were not correlated with sex, age, tumor size and tumor site in ESCC patients, but the overexpressions of mir-18a-5p, mir-23a-3p, mir-25-3p were significantly related to tumor differentiation (P<0.05), and a significant variation of mir-25-3p was found in different T stages (P<0.05). TGF-β1 level was lower in tumor tissues compared to normal tissues from the 52 ESCC patients, and higher in stage TIII/IV; than stage TI/II;(P<0.05), and a significant variation was found in histological differentiation (P<0.05). There was no correlation between mir-18a-5p, mir-23a-3p and TGF-β1, but mir-24-3p and mir-25-3p had an inverse correlation with TGF-β1 in tumor microenvironment of ESCC.

CONCLUSION: Mir-18a-5p, mir-23a-3p, mir-24-3p, mir-25-3p and TGF-β1 might play important roles in the carcinogenic process of ESCC.

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