JOURNAL ARTICLE

[Expression of odontogenic and osteogenic genes in bone marrow mesenchymal stem cells with overexpression of mouse dentin sialophosphoprotein]

Feng Zhu, Rong-Rong Nie, Ling Wu, Lei Liu, Wei Tang, Wei-Dong Tian
Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition 2008, 39 (2): 290-3
18630706

OBJECTIVE: To observe the overexpression of dentin sialophosphoprotein (DSPP) in mouse bone marrow mesenchymal stem cells (BM-MSC) and the expression of specific gene involved in odontogenic and osteogenic differentiation in transfected cells.

METHODS: Mouse bone marrow mesenchymal stem cells (BM-MSC) were cultured and then transfected with DSPP gene by adenovirus-mediated way in vitro. The transfection efficiency of DSPP gene was assessed by the marker gene green fluorescent protein (GFP). With having observed the morphological transformation of transfected BM-MSC, we studied whether the transfected BM-MSC would express odontogenic and osteogenic genes by RT-PCR.

RESULTS: The mouse BM-MSC were obtained and the adenovirus mediated DSPP gene used to transfect BM-MSC successfully. The transfected efficiency was 42.7%. The transfected BM-MSC were induced to differentiate into odontoblast-like cells and meanwhile expressed specific odontogenic and osteogenic differentiation genes such as DSPP, DMP1, Msx1/2, Pax9, Lhx6/7, Sox9, Cbfa1, Osx, Col I.

CONCLUSION: The bone marrow mesenchymal stem cells can make differentiation with the overexpression of dentin sialophosphoprotein and express specific odontogenic and osteogenic differentiation genes.

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