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English Abstract
Journal Article
Research Support, Non-U.S. Gov't
[Expression of odontogenic and osteogenic genes in bone marrow mesenchymal stem cells with overexpression of mouse dentin sialophosphoprotein].
Sichuan da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition 2008 March
OBJECTIVE: To observe the overexpression of dentin sialophosphoprotein (DSPP) in mouse bone marrow mesenchymal stem cells (BM-MSC) and the expression of specific gene involved in odontogenic and osteogenic differentiation in transfected cells.
METHODS: Mouse bone marrow mesenchymal stem cells (BM-MSC) were cultured and then transfected with DSPP gene by adenovirus-mediated way in vitro. The transfection efficiency of DSPP gene was assessed by the marker gene green fluorescent protein (GFP). With having observed the morphological transformation of transfected BM-MSC, we studied whether the transfected BM-MSC would express odontogenic and osteogenic genes by RT-PCR.
RESULTS: The mouse BM-MSC were obtained and the adenovirus mediated DSPP gene used to transfect BM-MSC successfully. The transfected efficiency was 42.7%. The transfected BM-MSC were induced to differentiate into odontoblast-like cells and meanwhile expressed specific odontogenic and osteogenic differentiation genes such as DSPP, DMP1, Msx1/2, Pax9, Lhx6/7, Sox9, Cbfa1, Osx, Col I.
CONCLUSION: The bone marrow mesenchymal stem cells can make differentiation with the overexpression of dentin sialophosphoprotein and express specific odontogenic and osteogenic differentiation genes.
METHODS: Mouse bone marrow mesenchymal stem cells (BM-MSC) were cultured and then transfected with DSPP gene by adenovirus-mediated way in vitro. The transfection efficiency of DSPP gene was assessed by the marker gene green fluorescent protein (GFP). With having observed the morphological transformation of transfected BM-MSC, we studied whether the transfected BM-MSC would express odontogenic and osteogenic genes by RT-PCR.
RESULTS: The mouse BM-MSC were obtained and the adenovirus mediated DSPP gene used to transfect BM-MSC successfully. The transfected efficiency was 42.7%. The transfected BM-MSC were induced to differentiate into odontoblast-like cells and meanwhile expressed specific odontogenic and osteogenic differentiation genes such as DSPP, DMP1, Msx1/2, Pax9, Lhx6/7, Sox9, Cbfa1, Osx, Col I.
CONCLUSION: The bone marrow mesenchymal stem cells can make differentiation with the overexpression of dentin sialophosphoprotein and express specific odontogenic and osteogenic differentiation genes.
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