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[The effect of interleukin-10 on neurocyte apoptosis in cerebral ischemia in rats].
Xi Bao Yu Fen Zi Mian Yi Xue za Zhi = Chinese Journal of Cellular and Molecular Immunology 2007 June
AIM: To investigate the effect of interleukin-10 on neurocyte apoptosis in cerebral ischemia in rats.
METHODS: 36 adult male Sprague-Dawley rats were randomly assigned into 3 groups: sham operation group, middle cerebral artery occlusion(MCAO) group and MCAO+interleukin-10 treatment group. Terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) staining was used to detect in situ cell apoptosis, immunohistochemical staining and RT-PCR were used to detect the expression of proapoptotic gene Fas, Fas ligand (FasL) and caspase-3 in peri-infarct, respectively.
RESULTS: Cerebral ischemia significantly induced neurocyte apoptosis and upregulated the expression of Fas, FasL and caspase-3, repcetively (P<0.05). IL-10 treatment significantly inhibited neurocyte apoptosis, and suppressed the expression of proapoptotic gene FasL and caspase-3, repcetively (P<0.05), but had no obvious effect on Fas expression (P>0.05).
CONCLUSION: IL-10 can suppress neurocyte apoptosis in cerebral ischemia, whose mechanisms seemed related to inhibiting the expression of proapoptotic gene FasL and caspase-3.
METHODS: 36 adult male Sprague-Dawley rats were randomly assigned into 3 groups: sham operation group, middle cerebral artery occlusion(MCAO) group and MCAO+interleukin-10 treatment group. Terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) staining was used to detect in situ cell apoptosis, immunohistochemical staining and RT-PCR were used to detect the expression of proapoptotic gene Fas, Fas ligand (FasL) and caspase-3 in peri-infarct, respectively.
RESULTS: Cerebral ischemia significantly induced neurocyte apoptosis and upregulated the expression of Fas, FasL and caspase-3, repcetively (P<0.05). IL-10 treatment significantly inhibited neurocyte apoptosis, and suppressed the expression of proapoptotic gene FasL and caspase-3, repcetively (P<0.05), but had no obvious effect on Fas expression (P>0.05).
CONCLUSION: IL-10 can suppress neurocyte apoptosis in cerebral ischemia, whose mechanisms seemed related to inhibiting the expression of proapoptotic gene FasL and caspase-3.
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