We have located links that may give you full text access.
English Abstract
Journal Article
[Influence of hypoxia on peroxisome proliferator activated receptor alpha and its mechanisms].
Chinese Journal of Tuberculosis and Respiratory Diseases 2003 September
OBJECTIVE: To study the expressions of peroxisome proliferator activated receptor alpha (PPAR-alpha) and hypoxia-inducible factor-1alpha (HIF-1alpha) during different hypoxia states in lung cancer cell A549, and to investigate the mechanism of HIF-1alpha induced PPARalpha expression.
METHODS: Firstly, lung cancer cells (A549) were divided into four groups: group A (control group); group B (hypoxia 24 h); group C (hypoxia 48 h); group D (hypoxia 72 h). The mRNA and protein of PPAR-alpha and HIF-1alpha were detected by RT-PCR and Western-blot on different groups. Secondly, the lung cancer cells (A549) were further divided into four groups: group E (control group); group F (antisense group); group G (sense group); group H (false-sense group). The influence of antisense oligonucleotide HIF-1alpha on PPAR-alpha was detected by RT-PCR and Western-blot.
RESULTS: Comparing with group A, the expressions of the mRNA and protein of PPAR-alpha and HIF-1alpha increased significantly in group B, and reached peak in group D. Conversely, the expressions of the mRNA and protein of PPAR-alpha were decreased after antisense oligonuclei of HIF-1alpha in group F, as compared with group E.
CONCLUSIONS: The expressions of the mRNA and protein of PPAR-alpha and HIF-1alpha are increased by hypoxia, and PPAR-alpha may be regulated by HIF-1alpha.
METHODS: Firstly, lung cancer cells (A549) were divided into four groups: group A (control group); group B (hypoxia 24 h); group C (hypoxia 48 h); group D (hypoxia 72 h). The mRNA and protein of PPAR-alpha and HIF-1alpha were detected by RT-PCR and Western-blot on different groups. Secondly, the lung cancer cells (A549) were further divided into four groups: group E (control group); group F (antisense group); group G (sense group); group H (false-sense group). The influence of antisense oligonucleotide HIF-1alpha on PPAR-alpha was detected by RT-PCR and Western-blot.
RESULTS: Comparing with group A, the expressions of the mRNA and protein of PPAR-alpha and HIF-1alpha increased significantly in group B, and reached peak in group D. Conversely, the expressions of the mRNA and protein of PPAR-alpha were decreased after antisense oligonuclei of HIF-1alpha in group F, as compared with group E.
CONCLUSIONS: The expressions of the mRNA and protein of PPAR-alpha and HIF-1alpha are increased by hypoxia, and PPAR-alpha may be regulated by HIF-1alpha.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app