Tariq Aziz, Muhammad Naveed, Muhammad Aqib Shabbir, Abid Sarwar, Ayaz Ali Khan, Ammarah Hasnain, Taqweem Ul Haq, Zhennai Yang, Abdellah Zinedine, João Miguel Rocha, Metab Alharbi
BACKGROUND: Lactiplantibacillus plantarum 12-3 holds great promise as a probiotic bacterial strain, yet its full potential remains untapped. This study aimed to better understand this potential therapeutic strain by exploring its genomic landscape, genetic diversity, CRISPR-Cas mechanism, genotype, and mechanistic perspectives for probiotic functionality and safety applications. METHODS: L. plantarum 12-3 was isolated from Tibetan kefir grains and, subsequently, Illumina and Single Molecule Real-Time (SMRT) technologies were used to extract and sequence genomic DNA from this organism...
April 11, 2024: Frontiers in Bioscience (Landmark Edition)