Andre Berndt, Justin Lee, Amanda Nguyen, Zheyu Jin, Aida Moghadasi, Chelsea Gibbs, Sarah Wait, Kira Evitts, Anthony Asencio, Samantha Bremner, Shani Zuniga, Vedant Chavan, Andy Williams, Annette Smith, Farid Moussavi-Harami, Michael Regnier, Jessica Young, David Mack, Elizabeth Nance, Patrick Boyle
H 2 O 2 is a key oxidant in mammalian biology and a pleiotropic signaling molecule at the physiological level, and its excessive accumulation in conjunction with decreased cellular reduction capacity is often found to be a common pathological marker. Here, we present a red fluorescent Genetically Encoded H 2 O 2 Indicator (GEHI) allowing versatile optogenetic dissection of redox biology. Our new GEHI, oROS-HT, is a chemigenetic sensor utilizing a HaloTag and Janelia Fluor (JF) rhodamine dye as fluorescent reporters...
April 17, 2024: Research Square