David Kim, Brandee Goo, Hong Shi, Philip Coffey, Praneet Veerapaneni, Ronnie Chouhaita, Nicole Cyriac, Ghaith Aboud, Stephen Cave, Jacob Greenway, Rohan Mundkur, Samah Ahmadieh, Ragheb Harb, Mourad Ogbi, David J Fulton, Yuqing Huo, Wei Zhang, Xiaochun Long, Avirup Guha, Ha Won Kim, Yang Shi, Robert D Rice, Dominic R Gallo, Vijay Patel, Richard Lee, Neal L Weintraub
INTRODUCTION: Human saphenous veins (SV) are widely used as grafts in coronary artery bypass (CABG) surgery but often fail due to neointima proliferation (NP). NP involves complex interplay between vascular smooth muscle cells (VSMC) and fibroblasts. Little is known, however, regarding the transcriptomic and proteomic dynamics of NP. Here, we performed multi-omics analysis in an ex vivo tissue culture model of NP in human SV procured for CABG surgery. METHODS AND RESULTS: Histological examination demonstrated significant elastin degradation and NP (indicated by increased neointima area and neointima/media ratio) in SV subjected to tissue culture...
November 17, 2023: bioRxiv