Biao Huang, Zipeng Zeng, Sunghyun Kim, Connor C Fausto, Kari Koppitch, Hui Li, Zexu Li, Xi Chen, Jinjin Guo, Chennan C Zhang, Tianyi Ma, Pedro Medina, Megan E Schreiber, Mateo W Xia, Ariel C Vonk, Tianyuan Xiang, Tadrushi Patel, Yidan Li, Riana K Parvez, Balint Der, Jyun Hao Chen, Zhenqing Liu, Matthew E Thornton, Brendan H Grubbs, Yarui Diao, Yali Dou, Ksenia Gnedeva, Qilong Ying, Nuria M Pastor-Soler, Teng Fei, Kenneth R Hallows, Nils O Lindström, Andrew P McMahon, Zhongwei Li
Nephron progenitor cells (NPCs) self-renew and differentiate into nephrons, the functional units of the kidney. Here, manipulation of p38 and YAP activity allowed for long-term clonal expansion of primary mouse and human NPCs and induced NPCs (iNPCs) from human pluripotent stem cells (hPSCs). Molecular analyses demonstrated that cultured iNPCs closely resemble primary human NPCs. iNPCs generated nephron organoids with minimal off-target cell types and enhanced maturation of podocytes relative to published human kidney organoid protocols...
April 22, 2024: Cell Stem Cell