Tiago H C De Bem, Alessandra Bridi, Haidee Tinning, Rafael Vilar Sampaio, Irene Malo-Estepa, Dapeng Wang, Elton J R Vasconcelos, Ricardo Perecin Nociti, Ana C F C M de Ávila, Juliano Rodrigues Sangalli, Igor Garcia Motta, Gilmar Arantes Ataíde, Júlio C B da Silva, Yeda Fumie Watanabe, Angela Gonella-Diaza, Juliano C da Silveira, Guilherme Pugliesi, Flávio Vieira Meirelles, Niamh Forde
We tested the hypothesis that the biosensor capability of the endometrium is mediated in part, by the effect of different cargo contained in the extracellular vesicles secreted by the conceptus during the peri-implantation period of pregnancy. We transferred Bos taurus taurus embryos of different origin, in vivo (high developmental potential (IV)), in vitro (intermediate developmental potential (IVF)), or cloned (low developmental potential (NT)), into Bos taurus indicus recipients. Extracellular vesicles (EVs) recovered from Day 16 conceptus-conditioned medium were characterized and their microRNA (miRNA) cargo sequenced alongside RNA sequencing of their respective endometria...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology