Boris Bonaventure, Antoine Rebendenne, Ana Luiza Chaves Valadão, Mary Arnaud-Arnould, Ségolène Gracias, Francisco Garcia de Gracia, Joe McKellar, Emmanuel Labaronne, Marine Tauziet, Valérie Vivet-Boudou, Eric Bernard, Laurence Briant, Nathalie Gros, Wassila Djilli, Valérie Courgnaud, Hugues Parrinello, Stéphanie Rialle, Mickaël Blaise, Laurent Lacroix, Marc Lavigne, Jean-Christophe Paillart, Emiliano P Ricci, Reiner Schulz, Nolwenn Jouvenet, Olivier Moncorgé, Caroline Goujon
Genome-wide screens are powerful approaches to unravel regulators of viral infections. Here, a CRISPR screen identifies the RNA helicase DDX42 as an intrinsic antiviral inhibitor of HIV-1. Depletion of endogenous DDX42 increases HIV-1 DNA accumulation and infection in cell lines and primary cells. DDX42 overexpression inhibits HIV-1 infection, whereas expression of a dominant-negative mutant increases infection. Importantly, DDX42 also restricts LINE-1 retrotransposition and infection with other retroviruses and positive-strand RNA viruses, including CHIKV and SARS-CoV-2...
September 26, 2022: EMBO Reports