Maria Panagopoulou, Makrina Karaglani, Konstantina Tzitzikou, Nikoleta Kessari, Konstantinos Arvanitidis, Kyriakos Amarantidis, George I Drosos, Spyros Gerou, Nikolaos Papanas, Dimitrios Papazoglou, Stavroula Baritaki, Theodoros C Constantinidis, Ekaterini Chatzaki
Circulating cell-free DNA (ccfDNA) of mitochondrial origin (ccf-mtDNA) consists of a minor fraction of total ccfDNA in blood or in other biological fluids. Aberrant levels of ccf-mtDNA have been observed in many pathologies. Here, we introduce a simple and effective standardized Taqman probe-based dual-qPCR assay for the simultaneous detection and relative quantification of nuclear and mitochondrial fragments of ccfDNA. Three pathologies of major burden, one malignancy (Breast Cancer, BrCa), one inflammatory (Osteoarthritis, OA) and one metabolic (Type 2 Diabetes, T2D), were studied...
April 10, 2024: International Journal of Molecular Sciences