Jon M Steichen, Ivy Phung, Eugenia Salcedo, Gabriel Ozorowski, Jordan R Willis, Sabyasachi Baboo, Alessia Liguori, Christopher A Cottrell, Jonathan L Torres, Patrick J Madden, Krystal M Ma, Henry J Sutton, Jeong Hyun Lee, Oleksandr Kalyuzhniy, Joel D Allen, Oscar L Rodriguez, Yumiko Adachi, Tina-Marie Mullen, Erik Georgeson, Michael Kubitz, Alison Burns, Shawn Barman, Rohini Mopuri, Amanda Metz, Tasha K Altheide, Jolene K Diedrich, Swati Saha, Kaitlyn Shields, Steven E Schultze, Melissa L Smith, Torben Schiffner, Dennis R Burton, Corey T Watson, Steven E Bosinger, Max Crispin, John R Yates, James C Paulson, Andrew B Ward, Devin Sok, Shane Crotty, William R Schief
Germline-targeting immunogens hold promise for initiating the induction of broadly neutralizing antibodies (bnAbs) to HIV and other pathogens. However, antibody-antigen recognition is typically dominated by heavy chain complementarity determining region 3 (HCDR3) interactions, and vaccine priming of HCDR3-dominant bnAbs by germline-targeting immunogens has not been demonstrated in humans or outbred animals. In this work, immunization with N332-GT5, an HIV envelope trimer designed to target precursors of the HCDR3-dominant bnAb BG18, primed bnAb-precursor B cells in eight of eight rhesus macaques to substantial frequencies and with diverse lineages in germinal center and memory B cells...
May 17, 2024: Science