Sarah Brunner, Marcus Höring, Gerhard Liebisch, Sabine Schweizer, Josef Scheiber, Piero Giansanti, Maria Hidrobo, Sven Hermeling, Josef Oeckl, Natalia Prudente de Mello, Fabiana Perocchi, Claudine Seeliger, Akim Strohmeyer, Martin Klingenspor, Johannes Plagge, Bernhard Küster, Ralph Burkhardt, Klaus-Peter Janssen, Josef Ecker
Lipid composition is conserved within sub-cellular compartments to maintain cell function. Lipidomic analyses of liver, muscle, white and brown adipose tissue (BAT) mitochondria revealed substantial differences in their glycerophospholipid (GPL) and free cholesterol (FC) contents. The GPL to FC ratio was 50-fold higher in brown than white adipose tissue mitochondria. Their purity was verified by comparison of proteomes with ER and mitochondria-associated membranes. A lipid signature containing PC and FC, calculated from the lipidomic profiles, allowed differentiation of mitochondria from BAT of mice housed at different temperatures...
August 2024: Life Science Alliance