Pilar Bustamante-Madrid, Antonio Barbáchano, David Albandea-Rodríguez, Javier Rodríguez-Cobos, Nuria Rodríguez-Salas, Isabel Prieto, Aurora Burgos, Jaime Martínez de Villarreal, Francisco X Real, José Manuel González-Sancho, María Jesús Larriba, Miguel Lafarga, Alberto Muñoz, Asunción Fernández-Barral
Understanding the mechanisms involved in colonic epithelial differentiation is key to unraveling the alterations causing inflammatory conditions and cancer. Organoid cultures provide an unique tool to address these questions but studies are scarce. We report a differentiation system toward enterocytes and goblet cells, the two major colonic epithelial cell lineages, using colon organoids generated from healthy tissue of colorectal cancer patients. Culture of these organoids in medium lacking stemness agents resulted in a modest ultrastructural differentiation phenotype with low-level expression of enterocyte (KLF4, KRT20, CA1, FABP2) and goblet cell (TFF2, TFF3, AGR2) lineage markers...
April 29, 2024: Cell Death & Disease