Junseong Park, Ilkyoo Koh, Junghwa Cha, Yoojung Oh, Jin-Kyoung Shim, Hyejin Kim, Ju Hyung Moon, Eui Hyun Kim, Jong Hee Chang, Pilnam Kim, Seok-Gu Kang
No standardized in vitro cell culture models for glioblastoma (GBM) have yet been established, excluding the traditional two-dimensional culture. GBM tumorspheres (TSs) have been highlighted as a good model platform for testing drug effects and characterizing specific features of GBM, but a detailed evaluation of their suitability and comparative performance is lacking. Here, we isolated GBM TSs and extracellular matrices (ECM) from tissues obtained from newly diagnosed IDH1 wild-type GBM patients and cultured GBM TSs on five different culture platforms: (1) ordinary TS culture liquid media (LM), (2) collagen-based three-dimensional (3D) matrix, (3) patient typical ECM-based 3D matrix, (4) patient tumor ECM-based 3D matrix, and (5) mouse brain...
April 19, 2024: Pharmaceuticals