Mahmood Yaseen Hachim, Noha Mousaad Elemam, Rakhee K Ramakrishnan, Laila Salameh, Ronald Olivenstein, Ibrahim Yaseen Hachim, Thenmozhi Venkatachalam, Bassam Mahboub, Saba Al Heialy, Rabih Halwani, Qutayba Hamid, Rifat Hamoudi
Background: Amphiregulin (AREG) expression in asthmatic airways and sputum was shown to increase and correlate with asthma. However, no studies were carried out to evaluate the AREG level in blood and saliva of asthmatic patients. Objective: To measure circulating AREG mRNA and protein concentrations in blood, saliva, and bronchial biopsies samples from asthmatic patients. Methods: Plasma and Saliva AREG protein concentrations were measured using ELISA while PBMCs, and Saliva mRNA expression was measured by RT qPCR in non-severe, and severe asthmatic patients compared to healthy controls...
2020: Frontiers in Medicine