Shantam Kalra, Amber Donnelly, Nishtha Singh, Daniel Matthews, Rafael Del Villar-Guerra, Victoria Bemmer, Cyril Dominguez, Natalie Allcock, Dmitry Cherny, Andrey Revyakin, David A Rusling
Here, we present a cross-linking approach to covalently functionalize and stabilize DNA origami structures in a one-pot reaction. Our strategy involves adding nucleotide sequences to adjacent staple strands, so that, upon assembly of the origami structure, the extensions form short hairpin duplexes targetable by psoralen-labeled triplex-forming oligonucleotides bearing other functional groups (pso-TFOs). Subsequent irradiation with UVA light generates psoralen adducts with one or both hairpin staples leading to site-specific attachment of the pso-TFO (and attached group) to the origami with ca...
May 2, 2024: Journal of the American Chemical Society