Masataka Shimonosono, Masaki Morimoto, Wataru Hirose, Yasuto Tomita, Norihiro Matsuura, Samuel Flashner, Mesra S Ebadi, Emilea H Okayasu, Christian Y Lee, William R Britton, Cecilia Martin, Beverly R Wuertz, Anuraag S Parikh, Uma M Sachdeva, Frank G Ondrey, Venkatram R Atigadda, Craig A Elmets, Julian A Abrams, Amanda B Muir, Andres J Klein-Szanto, Kenneth I Weinberg, Fatemeh Momen-Heravi, Hiroshi Nakagawa
BACKGROUND: Esophageal organoids from a variety of pathologies including cancer are grown in Advanced Dulbecco's Modified Eagle Medium-Nutrient Mixture F12 (hereafter ADF). However, the currently available ADF-based formulations are suboptimal for normal human esophageal organoids, limiting the ability to compare normal esophageal organoids with those representing a given disease state. METHODS: We have utilized immortalized normal human esophageal epithelial cell (keratinocyte) lines EPC1 and EPC2 and endoscopic normal esophageal biopsies to generate three-dimensional (3D) organoids...
May 22, 2024: bioRxiv