Guangdong Li, Laiqing Yan, Likai Wang, Wenkui Ma, Hao Wu, Shengyu Guan, Yujun Yao, Shoulong Deng, Hai Yang, Jinlong Zhang, Xiaosheng Zhang, Haixin Wu, Changjiu He, Pengyun Ji, Zhengxing Lian, Yingjie Wu, Lu Zhang, Guoshi Liu
Melatonin plays an important role in mammalian reproductive activities, to further understand the effects of endogenous melatonin on functions of ovary, the transgenic sheep with overexpression of melatonin synthetic enzyme gene ASMT in ovary were generated. The results showed that total melatonin content in follicular fluid of transgenic sheep was significantly greater than that in the wild type. Accordingly, the follicle numbers of transgenic sheep were also significantly greater than those in the WT. The results of follicular fluid metabolites sequencing showed that compared with WT, the differential metabolites of the transgenic sheep were significantly enriched in several signaling pathways, the largest number of metabolites was lipid metabolism pathway and the main differential metabolites were lipids and lipoid molecules...
April 24, 2024: International Journal of Biological Macromolecules