Ning Zhang, Damini Sood, Spencer C Guo, Nanhao Chen, Adam Antoszewski, Tegan Marianchuk, Archana Chavan, Supratim Dey, Yunxian Xiao, Lu Hong, Xiangda Peng, Michael Baxa, Carrie Partch, Lee-Ping Wang, Tobin R Sosnick, Aaron R Dinner, Andy LiWang
The oscillator of the cyanobacterial circadian clock relies on the ability of the KaiB protein to switch reversibly between a stable ground-state fold (gsKaiB) and an unstable fold-switched fold (fsKaiB). Rare fold-switching events by KaiB provide a critical delay in the negative feedback loop of this post-translational oscillator. In this study, we experimentally and computationally investigate the temperature dependence of fold switching and its mechanism. We demonstrate that the stability of gsKaiB increases with temperature compared to fsKaiB and that the Q10 value for the gsKaiB → fsKaiB transition is nearly three times smaller than that for the reverse transition...
May 21, 2024: bioRxiv