Hong Zhang, Mengshi Lei, Yu Zhang, Hao Li, Zhen He, Sheng Xie, Le Zhu, Shen Wang, Jianfeng Liu, Yan Li, Youming Lu, Cong Ma
At the synapse, presynaptic neurotransmitter release is tightly controlled by release machinery, involving the soluble N -ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins and Munc13. The Ca2+ sensor Doc2 cooperates with Munc13 to regulate neurotransmitter release, but the underlying mechanisms remain unclear. In our study, we have characterized the binding mode between Doc2 and Munc13 and found that Doc2 originally occludes Munc13 to inhibit SNARE complex assembly. Moreover, our investigation unveiled that EphB2, a presynaptic adhesion molecule (SAM) with inherent tyrosine kinase functionality, exhibits the capacity to phosphorylate Doc2...
May 17, 2024: Science Advances