Hyeon-Yeol Cho, Myungsik Yoo, Thanapat Pongkulapa, Hudifah Rabie, Alysson R Muotri, Perry T Yin, Jeong-Woo Choi, Ki-Bum Lee
The CRISPR-Cas9 technology has the potential to revolutionize the treatment of various diseases, including Rett syndrome, by enabling the correction of genes or mutations in human patient cells. However, several challenges need to be addressed before its widespread clinical application. These challenges include the low delivery efficiencies to target cells, the actual efficiency of the genome-editing process, and the precision with which the CRISPR-Cas system operates. Herein, the study presents a Magnetic Nanoparticle-Assisted Genome Editing (MAGE) platform, which significantly improves the transfection efficiency, biocompatibility, and genome-editing accuracy of CRISPR-Cas9 technology...
April 22, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)