Evelyn Susanto, Ana Marin Navarro, Leilei Zhou, Anders Sundström, Niek van Bree, Marina Stantic, Mohsen Moslem, Jignesh Tailor, Jonne Rietdijk, Veronica Zubillaga, Jens-Martin Hübner, Holger Weishaupt, Johanna Wolfsberger, Irina Alafuzoff, Ann Nordgren, Thierry Magnaldo, Peter Siesjö, John Inge Johnsen, Marcel Kool, Kristiina Tammimies, Anna Darabi, Fredrik J Swartling, Anna Falk, Margareta Wilhelm
Medulloblastoma is the most common malignant brain tumor in children. Here we describe a medulloblastoma model using Induced pluripotent stem (iPS) cell-derived human neuroepithelial stem (NES) cells generated from a Gorlin syndrome patient carrying a germline mutation in the sonic hedgehog (SHH) receptor PTCH1. We found that Gorlin NES cells formed tumors in mouse cerebellum mimicking human medulloblastoma. Retransplantation of tumor-isolated NES (tNES) cells resulted in accelerated tumor formation, cells with reduced growth factor dependency, enhanced neurosphere formation in vitro, and increased sensitivity to Vismodegib...
August 3, 2020: Proceedings of the National Academy of Sciences of the United States of America