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Sensitized Fluorescence

Zhen Li, Qingqing Yin, Binlong Chen, Zenghui Wang, Yue Yan, Tong Qi, Wei Chen, Qiang Zhang, Yiguang Wang
Photothermal therapy (PTT) has been recognized as a promising approach for cancer treatment due to its minimal invasiveness and low systemic side effects. However, developing a photothermal agent with accurate tumor imaging capability is a prerequisite for the efficient PTT. Here, we developed a series of ultra-pH-sensitive indocyanine green (ICG)-conjugated nanoparticles for fluorescence imaging-guided tumor PTT. These nanoparticles exhibited high fluorescence activation ratio (~100-fold) with sharp pH transition (ΔpHon/off <0...
February 11, 2019: Nanomedicine: Nanotechnology, Biology, and Medicine
Wei Zong, Wei Feng, Yun Jiang, Shaoqing Ju, Ming Cui, Rongrong Jing
Background Long non-coding RNAs (lncRNAs) have been reported to play a key role in gastric cancer (GC) tumorigenesis. However, the clinical application value of serum lncRNAs in GC has remained largely unknown. We investigated the role of a novel lncRNA named CTC-497E21.4 in the diagnosis and the prognosis of GC. Methods We focused on evaluation of lncRNA CTC-497E21.4 by real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR). The study involved following aspects: (1) confirmation of the higher lncRNA CTC-497E21...
January 7, 2019: Clinical Chemistry and Laboratory Medicine: CCLM
Birthe Stüven, Robert Stabel, Robert Ohlendorf, Julian Beck, Roman Schubert, Andreas Möglich
Cyclic nucleoside monophosphates (cNMP) serve as universal second messengers in signal transduction across prokaryotes and eukaryotes. As signaling often relies on transiently formed microdomains of elevated second messenger concentration, means to precisely perturb the spatiotemporal dynamics of cNMPs are uniquely poised for the interrogation of the underlying physiological processes. Optogenetics appears particularly suited as it affords light-dependent, accurate control in time and space of diverse cellular processes...
February 25, 2019: Biological Chemistry
Sheng Xie, Sesha Manuguri, Olof Ramström, Mingdi Yan
The fluorescence properties of AIE-active N-amidinated fluoroquinolones, efficiently obtained by a perfluoroaryl azide-aldehyde-amine reaction, have been studied. The fluorophores were discovered to elicit a highly sensitive fluorescence quenching response towards guest molecules with hydrogen-bond-donating ability. This effect was evaluated in a range of protic/aprotic solvents with different H-bonding capabilities, and also in aqueous media. The influence of acid/base was furthermore addressed. The hydrogen-bonding interactions were studied by IR, NMR, UV-Vis and time-resolved fluorescence decay, revealing their roles in quenching of the fluorescence emission...
February 14, 2019: Chemistry, An Asian Journal
Tianhai Lin, Hongyu Jin, Lina Gong, Ruichao Yu, Sheng Sun, Lu Yang, Peng Zhang, Ping Han, Jingqiu Cheng, Liangren Liu, Qiang Wei
BACKGROUND: To evaluate the diagnostic effectiveness and predictive value of fluorescence in situ hybridization (FISH) in the surveillance of non-muscle invasive bladder cancer (NMIBC). METHODS: PubMed/Medline, EMBASE, Web of Science, Ovid, Web of Knowledge, and Cochrane Library will be searched for studies related to the topic. The identification, inclusion, and exclusion flowcharts will be conducted according to preferred reporting items for systematic reviews and meta-analysis guidelines...
February 2019: Medicine (Baltimore)
Pawan Kumar, Ehsan Vahidzadeh, Ujwal Kumar Thakur, Piyush Kar, Kazi Mohammad Alam, Ankur Goswami, Najia Mahdi, Kai Cui, Guy M Bernard, Vladimir K Michaelis, Karthik Shankar
Modification of carbon nitride based polymeric 2D materials for tailoring their optical, electronic and chemical properties for various applications has gained significant interest. The present report demonstrates the synthesis of a novel modified carbon nitride framework with a remarkable 3:5 C:N stoichiometry (C3N5) and an electronic bandgap of 1.76 eV, by thermal deammoniation of melem hydrazine precursor. Characterization revealed that in C3N5 polymer, two s-heptazine units are bridged together with azo linkage, which constitutes an entirely new and different bonding fashion from g-C3N4 where three heptazine units are linked together with tertiary nitrogen...
February 14, 2019: Journal of the American Chemical Society
Christian Grundahl Frankær, Martin Rosenberg, Marco Santella, Kishwar J Hussain, Bo W Laursen, Thomas Just Sørensen
Since Sørensen and Bjerrum defined the pH scale, we have relied on two methods for determining pH, the colorimetric or the electrochemical. For pH electrodes, calibration is easy as a linear response is observed in the interesting pH range from 1 to ~12. For colorimetric sensors, the response follows the sigmoidal Bjerrum diagram of an acid-base equilibrium. Thus, calibration of colorimetric sensors is more complex. Here, seven pH responsive fluorescent dyes based on the same diazaoxatriangulenium (DAOTA) fluorophore linked to varying receptor groups were prepared...
February 14, 2019: ACS Sensors
Lirong Liu, Gangbing Zhu, Wei Zeng, Baohe Lv, Yinhui Yi
We present a new "off-on" fluorescence probe for detecting hypochlorite (ClO- ) based on silicon quantum dots coupled with silver nanoparticles (SiQDs/AgNPs) as nanocomplexes. Via introducing N-[3-(trimethoxysilyl)propyl]ethylenediamine and catechol as initial reactants, silicon quantum dots (SiQDs) with excellent properties were synthesized through a simple hydrothermal method. Transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy were used to characterize the morphology and structure of quantum dots...
February 14, 2019: Analytical and Bioanalytical Chemistry
Meltem Sertbas, Yasar Sertbas, Ozden Ezgi Uner, Serkan Elarslan, Nalan Okuroglu, Feyza Ak, Akin Dayan, Ali Ozdemır
INTRODUCTION Diabetes and its complications are a significant cause of morbidity and mortality. Advanced glycation endproducts (AGEs) play a major role in the pathogenesis of  diabetes complications. OBJECTIVES To investigate the possible uses of a biomicroscope (ClearPath DS-120) that shows age-adjusted lens fluorescence ratios (LFRs) for the diagnosis of diabetic peripheral neuropathy (DPN). PATIENTS AND METHODS One hundred and sixty type 2 diabetic patients who underwent LFR measurement were recruited to this study...
February 14, 2019: Polish Archives of Internal Medicine
Chaicharoen Tantanate, Ladawan Khowawisetsut, Kasama Sukapirom, Kovit Pattanapanyasat
The aim of this study was to evaluate the performance of automated impedance platelet counts by Beckman Coulter LH780 (PLT-LH), Sysmex XN-3000 (PLT-XNi) and fluorescence method by Sysmex XN-3000 (PLT-F) in patients with acute leukemia. Blood specimens were subjected to platelet measurements by evaluated methods and then compared against the international reference method (IRM). Eighty-two blood specimens were included. Bland-Altman plots of the differences between the evaluated methods and IRM demonstrated mean biases of PLT-LH, PLT-XNi and PLT-F of 9 × 109 /L, 11 × 109 /L and 2 × 109 /L, respectively...
February 14, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Sung Yong Byeon, Dong Ryun Lee, Kyoung Soo Yook, Jun Yeob Lee
The external quantum efficiency (EQE) of organic light-emitting diodes (OLEDs) has been dramatically improved by developing highly efficient organic emitters such as phosphorescent emitters and thermally activated delayed fluorescent (TADF) emitters. However, high-EQE OLED technologies suffer from relatively poor device lifetimes in spite of their high EQEs. In particular, the short lifetimes of blue phosphorescent and TADF OLEDs remain a big hurdle to overcome. Therefore, the high-EQE approach harvesting singlet excitons of fluorescent emitters by energy transfer processes from the host or sensitizer has been explored as an alternative for high-EQE OLED strategies...
February 13, 2019: Advanced Materials
Hudifah Rabie, Yixiao Zhang, Nicholas Pasquale, Maureen J Lagos, Philip E Batson, Ki-Bum Lee
Nondestructive neurotransmitter detection and real-time monitoring of stem cell differentiation are both of great significance in the field of neurodegenerative disease and regenerative medicine. Although luminescent biosensing nanoprobes have been developed to address this need, they have intrinsic limitations such as autofluorescence, scattering, and phototoxicity. Upconversion nanoparticles (UCNPs) have gained increasing attention for various biomedical applications due to their high photostability, low auto-fluorescent background, and deep tissue penetration; however, UCNPs also suffer from low emission intensities due to undesirable energy migration pathways...
February 13, 2019: Advanced Materials
Galina Nifontova, Fernanda Ramos-Gomes, Maria Baryshnikova, Frauke Alves, Igor Nabiev, Alyona Sukhanova
Imaging agents and drug carriers are commonly targeted toward cancer cell through functionalization with specific recognition molecules. Quantum dots (QDs) are fluorescent semiconductor nanocrystals whose extraordinary brightness and photostability make them attractive for direct fluorescent labeling of biomolecules or optical encoding of the membranes and cells. Here, we analyse the cytotoxicity of QD-encoded microcapsules, validate an approach to the activation of the microcapsule's surface for further functionalization with monoclonal antibody Trastuzumab, a humanized monoclonal antibody targeting the extracellular domain of the human epidermal growth factor receptor 2 (HER2) and already in clinical use for the treatment of HER2 positive breast cancer...
2019: Frontiers in Chemistry
Myung-Jin Lee, Jae-Sung Kwon, Heng Bo Jiang, Eun Ha Choi, Gyungsoon Park, Kwang-Mahn Kim
Titanium is commonly used as a biomaterial for dental implants. In this study, we investigated the antibacterial properties of titanium samples following treatment with a non-thermal atmospheric pressure plasma jet (NTAPPJ) on bacteria with two different cell wall structures, including gram-positive and gram-negative bacteria. The hydrophilicity and surface energy of titanium surfaces were significantly increased after NTAPPJ treatment without altering topographical features. Changes in the chemical composition and reductive potential were observed on the NTAPPJ-treated titanium surfaces...
February 13, 2019: Scientific Reports
Wenjing Li, Adam Dick, Fei Lu, Hui Zhang, Hong Sun
The proto-oncoprotein MET is a receptor tyrosine kinase that plays a key role in cancer cell growth and invasion. We have used fluorescence-tagged antibodies to activate MET in live serum-starved glioblastoma cells and monitor the fate of antibody-bound MET receptor in single cell-based assays. We found that the antibodies induced rapid and transient formation of highly polarized MET clusters on the plasma membrane and promoted the activation of MET, resembling the initial effects of binding to its ligand, HGF...
February 13, 2019: Scientific Reports
Elvira Román, Ioana Coman, Daniel Prieto, Rebeca Alonso-Monge, Jesús Pla
Clustered regularly interspaced short palindromic repeat (CRISPR) methodology is not only an efficient tool in gene editing but also an attractive platform to facilitate DNA, RNA, and protein interactions. We describe here the implementation of a CRISPR-based system to regulate expression in the clinically important yeast Candida albicans By fusing an allele of Streptococcus pyogenes Cas9 devoid of nuclease activity to a transcriptional repressor (Nrg1) or activator (Gal4), we were able to show specific repression or activation of the tester gene CAT1 , encoding the cytosolic catalase...
February 13, 2019: MSphere
Muzaffer A Bhat, Jai B Sharma, Kallol K Roy, Jayasree Sengupta, Debabrata Ghosh
BACKGROUND: Previous studies, which were primarily based on the fluorescent in-situ hybridisation (FISH) technique, revealed conflicting evidence regarding male foetal microchimerism in endometriosis. FISH is a relatively less sensitive technique, as it is performed on a small portion of the sample. Additionally, the probes used in the previous studies specifically detected centromeric and telomeric regions of Y chromosome, which are gene-sparse heterochromatised regions. In the present study, a panel of molecular biology tools such as qPCR, expression microarray, RNA-seq and qRT-PCR were employed to examine the Y chromosome microchimerism in the endometrium using secretory phase samples from fertile and infertile patients with severe (stage IV) ovarian endometriosis (OE) and without endometriosis...
February 13, 2019: Reproductive Biology and Endocrinology: RB&E
Sada Venkateswarlu, Saravanan Govindaraju, Roopkumar Sangubotla, Jongsung Kim, Min-Ho Lee, Kyusik Yun
The enormous ongoing industrial development has caused serious water pollution which has become a major crisis, particularly in developing countries. Among the various water pollutants, non-biodegradable heavy metal ions are the most prevalent. Thus, trace-level detection of these metal ions using a simple technique is essential. To address this issue, we have developed a fluorescent probe of Au/C nanodots (GCNDs-gold carbon nanodots) using an eco-friendly method based on an extract from waste onion leaves ( Allium cepa-red onions )...
February 12, 2019: Nanomaterials
S Munzi, C Branquinho, C Cruz, C Máguas, I D Leith, L J Sheppard, M A Sutton
Although it is generally accepted that δ15 N in lichen reflects predominating N isotope sources in the environment, confirmation of the direct correlation between lichen δ15 N and atmospheric δ15 N is still missing, especially under field conditions with most confounding factors controlled. To fill this gap and investigate the response of lichens with different tolerance to atmospheric N deposition, thalli of the sensitive Evernia prunastri and the tolerant Xanthoria parietina were exposed for ten weeks to different forms and doses of N in a field manipulation experiment where confounding factors were minimized...
February 25, 2019: Science of the Total Environment
Haoran Zhou, Erol Akçay, Brent R Helliker
Measurements of photosynthetic assimilation rate as a function of intercellular CO2 (A/Ci curves) are widely used to estimate photosynthetic parameters for C3 species, yet few parameters have been reported for C4 plants, because of a lack of estimation methods. Here, we extend the framework of widely used estimation methods for C3 plants to build estimation tools by exclusively fitting intensive A/Ci curves (6-8 more sampling points) for C4 using three versions of photosynthesis models with different assumptions about carbonic anhydrase processes and ATP distribution...
February 13, 2019: Photosynthesis Research
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