Cheng-Jung Ho, Huey-Jiun Ko, Tzu-Shao Liao, Xiang-Ren Zheng, Po-Hsu Chou, Li-Ting Wang, Ru-Wei Lin, Chung-Hwan Chen, Chihuei Wang
Apoptosis induced by doxorubicin, bortezomib, or paclitaxel, targeting DNA, 26S proteasome, and microtubules respectively, was assessed in two osteosarcoma cells, p53 wild-type U2OS and p53-null MG63 cells. Doxorubicin-induced apoptosis only occurred in U2OS, not in MG63. In contrast, bortezomib and paclitaxel could drive U2OS or MG63 toward apoptosis effectively, suggesting that apoptosis induced by bortezomib or paclitaxel is p53-independent. The expressions of Bcl2 family members such as Bcl2, Bcl-xl, and Puma could be seen in U2OS and MG63 cells with or without doxorubicin, bortezomib, or paclitaxel treatment...
October 4, 2021: Cell Death Discovery