Justas Žilinskas, Darius Stukas, Aldona Jasukaitienė, Jurgita Šapauskienė, Rasa Banienė, Sonata Trumbeckaitė, Saulius Švagždys, Marco Cicciu, Žilvinas Dambrauskas, Antanas Gulbinas, Algimantas Tamelis
PURPOSE: Treatment of advanced colorectal cancer (CRC) depends on the correct selection of personalized strategies. HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells) is a natural proteolipid milk compound that might serve as a novel cancer prevention and therapy candidate. Our purpose was to investigate HAMLET effect on viability, death pathway and mitochondrial bioenergetics of CRC cells with different KRAS/BRAF mutational status in vitro. METHODS: We treated three cell lines (Caco-2, LoVo, WiDr) with HAMLET to evaluate cell metabolic activity and viability, flow cytometry of apoptotic and necrotic cells, pro- and anti-apoptotic genes, and protein expressions...
April 26, 2023: Journal of Cancer Research and Clinical Oncology