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Fibroblast collagen

Marisa Tieger, Frederick A Jakobiec, Natalie Wolkow, Lina Ma, H Peggy Chang
We describe the clinical, histopathologic and immunohistochemical characteristics of episcleral/conjunctival pseudorheumatoid nodulosis, a new granulomatous entity that belongs among a group of related lesions. Specifically, pseudorheumatoid nodulosis should be differentiated from solitary rheumatoid nodules, rheumatoid nodulosis, accelerated rheumatoid nodules and nodulosis, and solitary pseudorheumatoid nodules. A 53-year-old man presented with bilateral painless, large, faintly yellow-grey, partially immobile, solid, circumscribed and occasionally confluent episcleral nodules of several months' duration...
February 14, 2019: Survey of Ophthalmology
Xiao Tian Wang, Claire C McKeever, Peter Vonu, Charles Patterson, Paul Y Liu
BACKGROUND: Wound contraction and re-epithelialization over the entire healing process had never been histologically examined daily in diabetic mouse wounds. Correlating morphological characters with molecular changes may be essential to understand the potential mechanism of impeded diabetic wound healing. MATERIALS AND METHODS: In 99 db/db and 63 db/m mice, dorsal-paired 8 mm-diameter wounds were created. Wound contraction and re-epithelialization were histologically analyzed daily-six wounds per group each day...
February 13, 2019: Journal of Surgical Research
Ewerton Zaniboni, Leonardo Bagne, Thaís Camargo, Maria Esméria Corezola do Amaral, Maira Felonato, Thiago Antônio Moretti de Andrade, Gláucia Maria Tech Dos Santos, Guilherme Ferreira Caetano, Marcelo Augusto Marreto Esquisatto, Milton Santamaria, Fernanda Aparecida Sampaio Mendonça
OBJECTIVES: Evaluate the bone remodeling during orthodontic movement with corticotomy when submitted to low-intensity electrical stimulation application (microcurrent-MC) and low-level laser therapy (LLLT). MATERIAL AND METHODS: One hundred and fifty Wistar rats were divided into the following 5 groups: (C) submitted to tooth movement; (Cort) tooth movement/corticotomy; (Cort-L) tooth movement/corticotomy/laser AsGaAl 808 nm (4.96J/50s); (Cort-Mc) tooth movement/corticotomy/microcurrent (10 μA/5 min); (Cort-L-Mc) tooth movement/corticotomy and laser/microcurrent alternated...
February 15, 2019: Clinical Oral Investigations
Jae Han Ko, Young Mi Kang, Jae Ho Yang, Ji Sup Kim, Won Jai Lee, Sang Ho Kim, Ick Hwan Yang, Seong Hwan Moon
PURPOSE: The aim of this study was to investigate the effects of relaxin (RLN) expression on fibrosis inhibition in synovial fibroblasts. MATERIALS AND METHODS: Tissue cells from patients with knee osteoarthritis and >30° flexion contractures were utilised. Synovial fibroblasts were activated by TGF-β1 (two nanograms per millilitre) and then exposed to Ad-RLN as a therapeutic gene, adenovirus-lacZ construct as a marker gene, and SB505124 as an inhibitor for TGF-β1 signal for 48 h...
February 13, 2019: Knee
Valentina Laghezza Masci, Anna-Rita Taddei, Thomas Courant, Ozgül Tezgel, Fabrice Navarro, Franco Giorgi, Denis Mariolle, Anna-Maria Fausto, Isabelle Texier
Curcumin-loaded collagen cryostructurates have been devised for wound healing applications. Curcumin displays strong antioxidant, antiseptic, and anti-inflammatory properties, while collagen is acknowledged for promoting cell adhesion, migration and differentiation. However, when curcumin is loaded directly into collagen hydrogels, it forms large molecular aggregates and clogs the matrix pores. A double-encapsulation strategy is therefore developed by loading curcumin into lipid nanoparticles (LNP), and embedding these particles inside collagen scaffolds...
February 15, 2019: Macromolecular Bioscience
Eran Nizri, Shoshi Bar-David, Asaf Aizic, Neta Sternbach, Guy Lahat, Ido Wolf, Joseph Klausner
OBJECTIVES: Pancreatic ductal adenocarcinoma (PDAC) is characterized by a peritumoral proliferation of fibroblasts and extracellular matrix production known as desmoplasia. We aimed to study desmoplasia in PDAC lymph node (LN) metastases. METHODS: We evaluated LNs from 66 patients with PDAC and LN metastases. We used immunohistochemistry and real-time polymerase chain reaction to phenotype the desmoplastic response. RESULTS: Desmoplasia was identified in 57% of patients with LN metastases (Des+)...
February 14, 2019: Pancreas
Ramkumar Mathur, Mahabub Maraj Alam, Xiao-Feng Zhao, Yuan Liao, Jeffrey Shen, Shannon Morgan, Tingting Huang, HwaJeong Lee, Edward Lee, Yunfei Huang, Xinjun Zhu
Intestinal fibrosis is an excessive proliferation of myofibroblasts and deposition of collagen, a condition frequently seen in Crohn's disease (CD). The mechanism underlying myofibroblast hyper-proliferation in CD needs to be better understood. In this report, we found that mTOR inhibitor rapamycin or mTOR deletion in CX3Cr1+ mononuclear phagocytes inhibits expression of interleukin (IL)-23, accompanied by reduced intestinal production of IL-22 and ameliorated fibrosis in the TNBS-induced fibrosis mouse model...
February 14, 2019: Mucosal Immunology
Vincent Huguier, Jean-Philippe Giot, Marie Simonneau, Pierre Levillain, Sandrine Charreau, Martine Garcia, Jean-François Jégou, Charles Bodet, Franck Morel, Jean-Claude Lecron, Laure Favot
Wound healing is a complex physiological process that repairs a skin lesion and produces fibrous tissue. In some cases, this process can lead to hypertrophic scars (HS) or keloid scars (KS), for which the pathophysiology remains poorly understood. Previous studies have reported the presence of oncostatin M (OSM) during the wound healing process; however, the role of OSM in pathological scarring remains to be precisely elucidated. This study aims to analyse the presence and involvement of OSM in the pathological scarring process...
February 14, 2019: Scientific Reports
Antje Schauer, Volker Adams, David M Poitz, Peggy Barthel, Dirk Joachim, Janet Friedrich, Axel Linke, Antje Augstein
BACKGROUND: The transcription factor Sox9 has been associated with cardiac injury and remodeling. Studies of mammalian hearts confirm Sox9 upregulation in fibroblasts following ischemic insults associated with enhanced fibrosis. The role of cardiomyocyte-specific Sox9 remains unclear. This study aimed to evaluate the role of cardiomyocyte-specific Sox9 in development and progression of left ventricular (LV) hypertrophy and fibrosis. METHODS: In male conditional Sox9 knockout mice (Sox9-KO) or floxed littermates (control group) transverse aortic constriction (TAC) was performed to induce LV hypertrophy...
February 2, 2019: International Journal of Cardiology
Fernando M Botelho, Rebecca Rodrigues, Jessica Guerette, Steven Wong, Dominik K Fritz, Carl D Richards
The accumulation of extracellular matrix in lung diseases involves numerous factors, including cytokines and chemokines that participate in cell activation in lung tissues and the circulation of fibrocytes that contribute to local fibrotic responses. The transient overexpression of the gp130 cytokine Oncostatin M can induce extracellular matrix (ECM) accumulation in mouse lungs, and here, we assess a role for IL-13 in this activity using gene deficient mice. The endotracheal administration of an adenovirus vector encoding Oncostatin M (AdOSM) caused increases in parenchymal lung collagen accumulation, neutrophil numbers, and CXCL1/KC chemokine elevation in bronchioalveolar lavage fluids...
February 5, 2019: Cells
Subhendu Mukherjee, Wei Sheng, Alexander Michkov, Krishna Sriarm, Rui Sun, Anna Dvorkin-Gheva, Paul A Insel, Luke J Janssen
We have shown that calcium (Ca2+ ) oscillations in human pulmonary fibroblasts (HPFs) contribute to pro-fibrotic effects of TGFβ and that disruption of these oscillations blunts features of pulmonary fibrosis. Prostaglandin E2 (PGE2 ) exerts anti-fibrotic effects in the lung but the mechanisms for this action are not well defined. We thus sought to explore interactions between PGE2 and the pro-fibrotic agent transforming growth factor beta (TGFβ) in pulmonary fibroblasts (PFs) isolated from patients with or without idiopathic pulmonary fibrosis (IPF)...
February 13, 2019: American Journal of Physiology. Lung Cellular and Molecular Physiology
Li Zhang, Li Zhang, Zhengquan Huang, Runlin Xing, Xiaochen Li, Songjiang Yin, Jun Mao, Nongshan Zhang, Wei Mei, Liang Ding, Peimin Wang
Fibroblast-like synoviocytes (FLSs) are the main effector cells of knee osteoarthritis (KOA) synovial fibrosis. Our last report showed that NLRP1 and NLRP3 inflammasomes may mediate LPS/ATP-induced FLSs pyroptosis in KOA. In the present study, we found an elevated hypoxia-inducible factor-1 α (HIF-1 α ) level in the synovial tissue of KOA model rats, and inhibiting the increase of HIF-1 α could improve synovial fibrosis in rats. Subsequently, we established LPS/ATP-induced model in FLSs mimicking the inflammatory environment of KOA...
2019: Oxidative Medicine and Cellular Longevity
Mohammed Albanna, Kyle W Binder, Sean V Murphy, Jaehyun Kim, Shadi A Qasem, Weixin Zhao, Josh Tan, Idris B El-Amin, Dennis D Dice, Julie Marco, Jason Green, Tao Xu, Aleksander Skardal, James H Holmes, John D Jackson, Anthony Atala, James J Yoo
The early treatment and rapid closure of acute or chronic wounds is essential for normal healing and prevention of hypertrophic scarring. The use of split thickness autografts is often limited by the availability of a suitable area of healthy donor skin to harvest. Cellular and non-cellular biological skin-equivalents are commonly used as an alternative treatment option for these patients, however these treatments usually involve multiple surgical procedures and associated with high costs of production and repeated wound treatment...
February 12, 2019: Scientific Reports
Asma Begum, Ross H McMillan, Yu-Tai Chang, Vesselin R Penchev, N V Rajeshkumar, Anirban Maitra, Michael G Goggins, James R Eshelman, Christopher L Wolfgang, Zeshaan A Rasheed, William Matsui
OBJECTIVE: Cancer-associated fibroblasts (CAFs) play an important role in the progression of pancreatic ductal adenocarcinoma (PDAC) by promoting tumor cell migration and drug resistance. We determined the impact of CAFs on PDAC cancer stem cells (CSCs). METHODS: Fibroblast cell lines from patients' tumors were cocultured with PDAC cells and examined for clonogenic growth and self-renewal using colony-forming assays and migration in vitro. Changes in the frequency of CSCs was determined by flow cytometry...
February 8, 2019: Pancreas
Nghi Dinh Huu, Sau Nguyen Huu, Xuan Le Thi, Thuong Nguyen Van, Phuong Pham Thi Minh, Trang Trinh Minh, Tam Hoang Van, Van Tran Cam, My Le Huyen, Khang Tran Hau, Marco Gandolfi, Francesca Satolli, Claudio Feliciani, Michael Tirant, Aleksandra Vojvodic, Torello Lotti
BACKGROUND: Keloid is an overactive condition of the skin tissue to early lesions characterised by proliferation of fibroblasts, excessive collagen production in the lesion. Treatment of keloids is a big challenge because of the poor response rate and high risk of recurrence after treatment. We found that bleomycin offers promise in the treatment of keloids. AIM: To evaluate the efficacy of bleomycin injected in the injury for keloids treatment. METHODS: The treatment was carried out in 55 patients having 120 keloids of different sizes and locations...
January 30, 2019: Open Access Macedonian Journal of Medical Sciences
Hasan Mete Aksoy, Berna Aksoy, Emel Çalikoglu
Systemic retinoids such as isotretinoin and acitretin are associated with numerous side effects but are therapeutically valuable in dermatological practice. Several studies have reported the wound-healing effects of treatment with concurrent systemic retinoids in addition to surgical treatments. Herein, we describe two patients with scar dehiscence that developed after initiation of systemic retinoid treatment following cutaneous surgery. In Case 1, isotretinoin was thought to decrease collagen production to a greater degree than did degradation during the wound remodeling phase, which resulted in wound dehiscence...
January 2019: Indian Journal of Dermatology
Kenji Kawabe, Takeshi Takarada
Mesenchymal stem cell (MSC) is a type of tissue stem cell. In clinical studies, cultured MSCs have shown important therapeutic effects on diseases via the reduction of neurological defects and regulation of immune responses. However, in vivo MSC localization, function, and properties are poorly understood; therefore, the molecular understanding of MSCs hierarchy is less advanced compared to hematopoietic stem cell hierarchy. To address these issues, we developed a method that enables us to visualize MSCs, manipulate their function, and analyze their molecular biology in vivo...
2019: Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica
Yifan Zhang, Jing Wang, Sizheng Zhou, Zhibo Xie, Chuandong Wang, Ya Gao, Jia Zhou, Xiaoling Zhang, Qingfeng Li
Skin fibrosis is mainly characterized by excessive collagen deposition. Studies have recently identified a number of flavonoids with variable structures that have the potency of inhibiting collagen synthesis and thus attenuating organ fibrosis. In this study, we found that flavones with 5, 7, 3', 4' hydroxy substitution reduced collagen expression most efficiently. Among those flavones, luteolin, quercetin, and myricetin were selected for follow-up. In vivo, the three compounds ameliorated skin fibrosis and reduced collagen deposition...
February 11, 2019: Cell Death & Disease
Aina He, Wenqian Fang, Kun Zhao, Yajun Wang, Jie Li, Chongzhe Yang, Feriel Benadjaoud, Guo-Ping Shi
Mast cells (MCs) have been implicated in the pathogenesis of cardiometabolic diseases by releasing pro-inflammatory mediators. Patients and animals with diabetic cardiomyopathy (DCM) also show inflammatory cell accumulation in the heart. Here, we detected MCs in mouse heart after streptozotocin (STZ)-induced DCM. DCM production caused significant systole and diastole interventricular septum and left ventricular (LV) posterior wall thinning, and systolic LV internal dilation in wild-type (WT) mice. DCM production also led to significant reductions of fractional shortening percentage, heart rate, body weight, heart weight, and significant increases of kidney, pancreas, and lung weight to body weight ratios, and blood hemoglobin HbA1c and glucose levels in WT mice...
January 19, 2019: Translational Research: the Journal of Laboratory and Clinical Medicine
Jia Wang, Wei Shen, Jun-Yan Zhang, Chang-Hao Jia, Mei-Lin Xie
Stevioside, a natural glycoside compound, has many beneficial biological activities, but its protective effect on myocardial fibrosis has not been reported yet. This study aimed to investigate the effect of stevioside. The isoproterenol-induced model mice were orally given stevioside 75-300 mg kg-1 for 40 days. The results showed that after the administration of stevioside, the myocardial hydroxyproline, collagen accumulation, and protein expressions of collagen I/III, α-smooth muscle actin, transforming growth factor-β1 (TGF-β1), nuclear factor kappa B p65 (NF-κB p65), Smad2/3, and P-Smad2/3 were decreased, while the glutathione peroxidase and superoxide dismutase levels in serum and myocardial tissues and protein expressions of myocardial peroxisome proliferator-activated receptor γ (PPARγ) and Smad7 were increased...
February 8, 2019: Food & Function
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