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Oocyte retrieval

Ali Sami Gurbuz, Ali Cenker
Transvaginal oocyte retrieval is a crucial step in assisted reproductive technology. Various complications may arise during this procedure. Ureteral injury is a rare, but a serious complication in gynecological practice. During oocyte retrieval, ureteral injuries, detachment and obstruction can be seen, though rare. In this study, we will present ureteral obstruction that develops secondary to small hematoma, which mimics ovarian cyst torsion or ruptured ovarian cyst.
February 15, 2019: International Braz J Urol: Official Journal of the Brazilian Society of Urology
Mingyang Li, Lihua Yao, Meizhen Xin, Minzhi Gao
AIM: To analyze the effects of high ovarian response on endometrial collagen synthesis and related gene expression during the peri-implantation period in patients undergoing in vitro fertilization-embryo transfer. METHODS: Peripheral blood and endometrial biopsies were obtained from infertile women on day 6 after oocytes retrieval or ovulation in 16 stimulated cycles (SC) and 16 natural cycles (NC) respectively. Serum estrogen (E2 ), progesterone (P4 ), histological staging, endometrial collagen, matrix metalloproteinases (MMP2, 9) and tissue inhibitors of metalloproteinases (TIMP1, 3) were assayed...
February 18, 2019: Journal of Obstetrics and Gynaecology Research
Ping Yuan, Ruiqi Li, Di Li, Lingyan Zheng, Songbang Ou, Haijing Zhao, Qingxue Zhang, Wenjun Wang
PURPOSE: Empty follicle syndrome (EFS) is a complex reproductive disorder characterized by the repeated failure to aspirate oocytes from mature ovarian follicles during in vitro fertilization (IVF). In addition to some cases caused by iatrogenic problems and known genetic factors, there are still many unexplained aspects of EFS. Here, we aimed to assess the clinical and genetic characteristics of two EFS patients. METHODS: We have characterized two primary infertility patients with EFS in a nonconsanguineous family from China...
February 18, 2019: Journal of Assisted Reproduction and Genetics
Leili Safdarian, Seyedeh Noushin Ghalandarpoor Attar, Ashraf Aleyasin, Marzieh Aghahosseini, Fateme Sadaf Sarfjoo, Sedigheh Hosseinimousa
Background: Endometriosis, can cause ovarian conflict and reduced ovarian reserve that could lead to lower response to assisted reproductive techniques. Objective: Current study was conducted to determine the association between level of anti-mullerian hormone (AMH) and the infertility treatment outcomes in infertile females with endometriosis versus the non-endometriosis infertile subject. Materials and Methods: In this case-control study, 64 infertile females who referred to Shariati Hospital from April 2015 to November 2017 were enrolled...
November 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
Pedro Montoya-Botero, Nikolaos P Polyzos
Controlled ovarian hyperstimulation (COH) is a crucial part of assisted reproductive technologies (ART) that resulted in a substantial increase in pregnancy rates from in vitro fertilization (IVF). However, in spite of the apparent benefit of COH on an increase in the number of follicles and the number of oocytes retrieved, allowing for extended embryo culture and enabling the selection of the best quality embryo for transfer, several reports has shown that the supraphysiologic hormonal levels may indeed have a detrimental effect at the endometrial level...
September 13, 2018: Best Practice & Research. Clinical Endocrinology & Metabolism
Jing Zhuang, Hengli Li, Xiaohong Li, Dongmei Tian, Dan Yang, Minghui Zhu
Unexpected poor ovarian response (UPOR) is a problem for both clinicians and infertile couples, because our understanding of this situation is limited. This article investigated incidence of UPOR in women <35 years with normal ovarian reserve function with further analysis.This is a retrospective study, which included 567 women who accepted their first IVF-ET/ICSI. Based on the number of oocytes retrieved, clinic pregnancy rate of fresh cycle, and cycle cancellation rate of fresh cycle, the included cycles were divided into three groups namely unexpected poor ovarian response (UPOR) (n = 120), for which number of oocytes retrieved was not more than 6; normal ovarian response (NOR) (n = 223), for which number of oocytes retrieved was between 7 and 12; and unexpected high ovarian response (UHOR) (n = 224), for which the number of oocytes retrieved was 13 at least...
February 2019: Medicine (Baltimore)
Mariana Carlos Alves, Andreia Leitão Marques, Helena Barros Leite, Ana Paula Sousa, Teresa Almeida-Santos
INTRODUCTION: Medically assisted reproduction in natural cycle has been investigated, especially in women with poor response to conventional ovarian stimulation, with endometrial receptivity improvement, lower cost and possibility of successive cycles. The disadvantages are: lower profitability per treatment cycle and higher cancellation rate. The aim of this study was to determine the rate of clinical pregnancy in infertile women subjected to medically assisted reproduction in natural cycle...
February 1, 2019: Acta Médica Portuguesa
Zeynep Ozturk Inal, Yaprak Engin Ustun, Nafiye Yilmaz, Ayla Aktulay, Yesim Bardakci, Cavidan Gulerman
In this study, our aim was to determine which factor is more correlated with the number of oocytes retrieved in patients with endometrioma compared with controls undergoing in vitro fertilisation-intracytoplasmic sperm injection (IVF-ICSI) cycles: antral follicle count (AFC) or anti-Müllerian hormone (AMH). A total of 60 women with endometrioma and a control population of 60 women without endometrioma in the same period were randomly selected underwent the injection IVF-ICSI treatment. No significant differences were found between the groups in terms of age (28...
February 11, 2019: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
Edson Borges, Bianca Ferrarini Zanetti, Daniela Paes de Almeida Ferreira Braga, Amanda Souza Setti, Rita Cássia Sávio Figueira, Assumpto Iaconelli
OBJECTIVE: To study which factors affect perinatal outcomes in intracytoplasmic sperm injection (ICSI) cycles. METHODS: Data was obtained from 402 live births born to 307 patients undergoing ICSI cycles in a private university-affiliated IVF center between Jan/2014 and Dec/2015. The influences of the cycles' characteristics on the number of gestational weeks to livebirth (GW), baby birth weight (BW), and baby birth length (BL) were evaluated by linear regression models, adjusted for maternal age and body mass index, number of transferred embryos, number of gestational sacs, and number of born infants...
February 12, 2019: JBRA Assisted Reproduction
Fazilet Kubra Boynukalin, Munevver Serdarogullari, Meral Gultomruk, Onder Coban, Necati Findikli, Mustafa Bahceci
The aim of this study was to evaluate the possible effects of endometriosis on early embryo development, by comparing the morphokinetic development of embryos obtained from women with clinically confirmed endometriosis with the ones obtained from tubal factor infertility cases. A total of 82 cycles/patients including 53 cycles with endometriosis and 29 cycles with tubal factor infertility were evaluated. A total of 439 embryos were scored for embryo morphokinetics. Age, body mass index, fertilization rates were similar within the groups...
February 11, 2019: Systems Biology in Reproductive Medicine
Antonis Makrigiannakis, Thomas Vrekoussis, Fanourios Makrigiannakis, Halil Ruso, Sophia N Kalantaridou, Timur Gurgan
BACKGROUND: The intra-uterine administration of activated autologous peripheral blood monocytes (PBMC) prior to embryo transfer seems to improve reproductive outcomes in women with repeated implantation failure (RIF). We have previously shown that the intrauterine administration of PBMC treated with corticotropin releasing hormone (CRH) prior to blastocyst transfer (day 5), improves significantly the clinical pregnancy rate of women with RIF. In the present cross-over pilot study, we have investigated whether CRH-PBMC treatment could be of benefit in case of fresh early cleavage stage embryo transfer (day 3) in women with RIF...
February 9, 2019: European Journal of Clinical Investigation
Haibin Zhao, Wenrong Tao, Mei Li, Hui Liu, Keliang Wu, Shuiying Ma
PURPOSE: To compare the effectiveness of two protocols of blastocyst biopsy submitted to preimplantation genetic testing for aneuploidies (PGT-A). METHODS: This is a randomized controlled trial of a cohort of 221 patients undergoing PGT-A. 106 female patients aged ≤ 40 years with no less than 8 mature oocytes retrieved and ≥ 3 good-quality embryos on day 3 were randomly assigned to the day-3 hatching-based TE biopsy. The remaining 115 females aged ≤ 40 years with ≥ 8 MII oocytes obtained and no less than 3 high-quality embryos on day 3 were assigned to the TE biopsy without hatching group (also called the new biopsy group)...
February 8, 2019: Archives of Gynecology and Obstetrics
Marzie Farimani, Safoura Heshmati, Jalal Poorolajal, Maryam Bahmanzadeh
The prevalence of poor response to gonadotropin stimulation is approximately 9-24% in women undergoing in vitro fertilization. Interestingly, due to containing a variety of growth factors, platelet-rich plasma (PRP) can play an important role in tissue regeneration and healing. Thus, in this research, we aimed to investigate the intra-ovarian injection of PRP in women with poor ovarian response. To this goal, 23 poor responders constituted the study population, from among whom 19 women were enrolled. These patients underwent ovarian stimulation according to the Shanghai protocol...
February 5, 2019: Molecular Biology Reports
Min Zheng, Gaoge Zuo, Jing Tong, Zi-Jiang Chen, Wei-Ping Li, Cong Zhang
PURPOSE: Melatonin is an important factor in regulating numerous processes in human female reproduction. The aim of the present study was to compare melatonin levels in the follicular fluid (FF) of ovarian hyperstimulation syndrome (OHSS) women with those of non-OHSS women undergoing in vitro fertilization (IVF)-embryo transfer and to evaluate the relationship between FF melatonin levels and IVF outcomes in these women. METHODS: We determined FF melatonin levels in 20 OHSS women and 23 non-OHSS women on oocyte retrieval day...
February 6, 2019: Archives of Gynecology and Obstetrics
Kenny A Rodriguez-Wallberg, Anna Marklund, Frida Lundberg, Ida Wikander, Milan Milenkovic, Amandine Anastacio, Fotios Sergouniotis, Kjell Wånggren, Jeanette Ekengren, Tekla Lind, Birgit Borgström
INTRODUCTION: In Scandinavian countries, programs for fertility preservation are offered free of charge at tertiary-care university hospitals to all patients facing treatments with risk of subsequent sterility. In this prospective study we aimed to investigate trends in female patients' choices after counseling and fertility preservation outcomes during follow-up in relation to benign vs malignant indications. MATERIAL AND METHODS: Data on 1254 females including 1076 adults and 178 girls who received fertility preservation counseling for either oncologic (N=852) or benign indications (N=402) at Karolinska University Hospital, Stockholm, between 1 Oct 1998 and 1 Dec 2018 were analyzed...
February 5, 2019: Acta Obstetricia et Gynecologica Scandinavica
Carmen Rubio, Lorena Rodrigo, Carmen Garcia-Pascual, Vanessa Peinado, Inmaculada Campos-Galindo, Sandra Garcia-Herrero, Carlos Simón
We review here the evolution in the field of embryo aneuploidy testing over the last 20 years, from the analysis of a subset of chromosomes by fluorescence in situ hybridisation (FISH) to the transition toward a more comprehensive analysis of all 24 chromosomes. This current comprehensive aneuploidy testing most commonly employs next-generation sequencing (NGS). We present our experience in over 130,000 embryo biopsies using this technology. The incidence of aneuploidy was lower in trophectoderm biopsies compared to cleavage-stage biopsies...
February 4, 2019: Biology of Reproduction
Qifan Zhang, Shibo Sun, Chen Zhu, Yujian Zheng, Qing Cai, Xiaolu Liang, Haorong Xie, Jie Zhou
The aim of the present study was to identify the differentially expressed genes (DEGs) between primary tumor tissue and adjacent non‑tumor tissue of hepatocellular carcinoma (HCC) samples in order to investigate the mechanisms of HCC. The microarray data of the datasets GSE76427, GSE84005 and GSE57957 were downloaded from the Gene Expression Omnibus database. DEGs were identified using the limma package in the R programming language. Following the intersection of the DEGs screened from the three datasets, 218 genes were selected for further study...
February 4, 2019: Molecular Medicine Reports
Yang Xu, Yu-Song Zhang, Dong-Yi Zhu, Xiang-Hong Zhai, Feng-Xia Wu, An-Cong Wang
The aim of the present study was to evaluate the influence of a gonadotropin-releasing hormone (GnRH) antagonist compared with a GnRH agonist on in vitro fertilization (IVF) cycle outcome in reproductive women. The characteristics of treatment and outcomes of pregnancy were retrospectively compared between the antagonist (GnRH-A, antagonist group) and agonist (GnRH-a, agonist group) regimens. The area under the curve (AUC) of receiver operating characteristic (ROC) curves was also used to evaluate whether the endometrial thickness (cm), progesterone (P) level (ng/ml) and estradiol (E2 ) level (pg/ml) on the day of human chorionic gonadotropin (hCG) administration (hCG day) had the ideal sensitivity and specificity for predicting clinical pregnancy...
February 2019: Biomedical Reports
Clara Q Wu, Arianne Albert, Sukainah Alfaraj, Omur Taskin, Ghadeer M Alkusayer, Jon Havelock, Paul Yong, Catherine Allaire, Mohamed A Bedaiwy
Controversy exists regarding surgical management of endometriomas in infertile women before in vitro fertilization (IVF) because growing evidence indicates that surgery may impair the ovarian response. The objective of the present systematic review and meta-analysis was to compare surgical and expectant management of endometriomas regarding IVF outcomes. Prospective and retrospective controlled studies were found via the Cochrane Library, Embase, and MEDLINE databases. Thirteen studies (1 randomized controlled trial and 12 observational studies, N = 2878) were pooled, and similar live birth rates were observed in the surgically and expectantly managed groups (odds ratio = 0...
February 2019: Journal of Minimally Invasive Gynecology
Milan D Perovic, Emina M Sudar-Milovanovic, Ema D Simonovic, Ivana M Resanovic, Veselin D Draganic, Jovana D Radakovic, Ivan A Soldatovic, Esma R Isenovic
Controlled ovarian stimulation (COS) is used to augment the number of retrieved oocytes in in vitro fertilization (IVF). Follicular fluid (FF) contributes significantly to oocyte quality. Since the FF is composed of follicular secretions and plasma exudation, it reflects alterations in granulosa and thecal cells secretion as well as changes in the level of plasma constituents. Phospholipids (PL) and free fatty acids (FFA) are important constituents of both, FF and serum. Our hypothesis is that COS affects the level of PL and FFA in serum...
February 2019: Medical Hypotheses
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