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Hongran Yin, Hongzhi Wang, Zhefeng Li, Dan Shu, Peixuan Guo
Displaying the advantage of nanoparticles in cancer targeting and drug delivery, micelles have shown great potential in cancer therapy. The mechanism for micelle targeting to cancer without the need of ligand is due to the size advantage of micelles within the lower end of the nanometer scale that is the optimal size favoring Enhanced Permeability and Retention (EPR) effect while escaping trapping by macrophages. MicroRNAs (miRNAs) are ubiquitous and play critical roles in regulating gene expression, cell growth and cancer development...
December 13, 2018: ACS Nano
Shanthi Sabarimurugan, Madhav Madurantakam Royam, Ankita Das, Shrestha Das, Gothandam K M, Rama Jayaraj
BACKGROUND: Melanoma is the most aggressive and deadly form of skin cancer. The molecular variability involving microRNA (miRNA) expression plays a significant role in melanogenesis, which leads to poor prognostic effects in melanoma. Since there is a scarcity of comprehensive data on the prognostic role of miRNAs in melanoma patients, this study focuses on filling this knowledge gap through a systematic review and meta-analysis. METHODS: The included studies were extracted from several bibliographic databases between 2012 and 2018 using multiple keywords according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines...
December 2018: Molecular Diagnosis & Therapy
Qingxin Liu, Jialong Fan, Chuang Zhou, Liqun Wang, Bin Zhao, Haibin Zhang, Bin Liu, Chunyi Tong
As a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer. With the tool of molecular beacons which can hybridize specifically with target miRNA-21 and generate fluorescence signal change, this paper develops a direct, simple, and rapid method for miRNA-21 detection with detection limit of 0.5 nM. Under the optimal conditions, the method was used to detect the expression of miRNA-21 in tumor cells and tissues...
2018: International Journal of Analytical Chemistry
Tingting Fan, Yu Mao, Qinsheng Sun, Feng Liu, Jin-Shun Lin, Yajie Liu, Junwei Cui, Yuyang Jiang
Serum circulating microRNAs (c-miRNAs) are serving as useful biomarkers for cancer diagnosis. Here, we describe the development of a one-step branched rolling circle amplification (BRCA) method to measure serum c-miRNAs levels for early diagnosis of breast cancer. Four c-miRNAs, c-miRNA16 (c-miR-16), c-miRNA21 (c-miR-21), c-miRNA155 (c-miR-155), and c-miRNA195 (c-miR-195) were isolated from the serum of 49 breast cancer patients (stages I-IV) and 19 healthy controls, and analyzed using one-step BRCA. The serum levels of c-miR16, c-miR21, c-miR155, and c-miR195 were higher (P < 0...
September 2018: Cancer Science
Iacopo Fabiani, Nicola Riccardo Pugliese, Enrico Calogero, Lorenzo Conte, Maria Chiara Mazzanti, Cristian Scatena, Claudia Scopelliti, Elena Tantillo, Matteo Passiatore, Marco Angelillis, Giuseppe Antonio Naccarato, Rossella Di Stefano, Anna Sonia Petronio, Vitantonio Di Bello
Aortic valve stenosis (AVS) represents a cluster of different phenotypes, considering gradient and flow pattern. Circulating micro RNAs may reflect specific pathophysiological processes and could be useful biomarkers to identify disease. We assessed 80 patients (81, 76.7-84 years; 46, 57.5%females) with severe AVS. We performed bio-humoral evaluation (including circulating miRNA-1, 21, 29, 133) and 2D-echocardiography. Patients were classified according to ACC/AHA groups (D1-D3) and flow-gradient classification, considering normal/low flow, (NF/LF) and normal/high gradient, (NG/HG)...
July 2, 2018: Scientific Reports
Y Cai, L Zhan, C Z Zhang, X D Wang, D B Lu, L B Cheng, H L Xu, X S Wang
No abstract text is available yet for this article.
February 20, 2018: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
Oana Milas, Florica Gadalean, Adrian Vlad, Victor Dumitrascu, Cristina Gluhovschi, Gheorghe Gluhovschi, Silvia Velciov, Roxana Popescu, Flaviu Bob, Petru Matusz, Agneta-Maria Pusztai, Octavian M Cretu, Alina Secara, Anca Simulescu, Sorin Ursoniu, Daliborca Vlad, Ligia Petrica
MicroRNAs (miRNAs) are short non-coding RNA species that are important post-transcriptional regulators of gene expression. The aim of the study was to establish a potential explanation of podocyte damage and proximal tubule (PT) dysfunction induced by deregulated miRNAs expression in the course of type 2 diabetes mellitus (DM). A total of 68 patients with type 2 DM and 11 healthy subjects were enrolled in a cross-sectional study and assessed concerning urinary albumin:creatinine ratio (UACR), urinary N -acetyl-β-D-glucosamininidase (NAG), urinary kidney injury molecule-1, urinary nephrin, podocalyxin, synaptopodin, estimated glomerular filtration rate (eGFR), urinary miRNA21, miRNA124, and miRNA192...
April 2018: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
Andrea Lampis, Pietro Carotenuto, Georgios Vlachogiannis, Luciano Cascione, Somaieh Hedayat, Rosemary Burke, Paul Clarke, Else Bosma, Michele Simbolo, Aldo Scarpa, Sijia Yu, Rebecca Cole, Elizabeth Smyth, Javier Fernández Mateos, Ruwaida Begum, Blanka Hezelova, Zakaria Eltahir, Andrew Wotherspoon, Nicos Fotiadis, Maria Antonietta Bali, Chirag Nepal, Khurum Khan, Mark Stubbs, Jens C Hahne, Pierluigi Gasparini, Vincenza Guzzardo, Carlo M Croce, Suzanne Eccles, Matteo Fassan, David Cunningham, Jesper B Andersen, Paul Workman, Nicola Valeri, Chiara Braconi
BACKGROUND & AIMS: Cholangiocarcinomas (CCA) are resistant to chemotherapy, so new therapeutic agents are needed. We performed a screen to identify small-molecule compounds that are active against CCAs. Levels of microRNA 21 (MIR21 or miRNA21) are increased in CCAs. We investigated whether miRNA21 mediates resistance of CCA cells and organoids to HSP90 inhibitors. METHODS: We performed a high-throughput screen of 484 small-molecule compounds to identify those that reduced viability of 6 human CCA cell lines...
March 2018: Gastroenterology
Dongdong Zeng, Zehua Wang, Zhiqiang Meng, Peng Wang, Lili San, Wei Wang, Ali Aldalbahi, Li Li, Juwen Shen, Xianqiang Mi
Specific and sensitive biomarker detection is essential to early cancer diagnosis. In this study, we demonstrate an ultrasensitive electrochemical biosensor with the ability to detect multiple pancreatic carcinoma (PC)-related microRNA biomarkers. By employing DNA tetrahedral nanostructure capture probes to enhance the detection sensitivity as well as a disposable 16-channel screen-printed gold electrode (SPGE) detection platform to enhance the detection efficiency, we were able to simultaneously detect four PC-related miRNAs: miRNA21, miRNA155, miRNA196a, and miRNA210...
July 19, 2017: ACS Applied Materials & Interfaces
Shanaya Patel, Rakesh Rawal
Late diagnosis, low therapeutic response, and metastasis are accountable for poor 5-year survival rate of OSCC. These failures are attributed to the existence of "cancer stem cell (CSC)" subpopulation. Hence, it is necessary to identify and understand the mechanism of CSCs in tumor development, metastasis, and chemotherapeutic response. Propelling evidences suggest that microRNA (miRNA)-mediated regulation and cytokines of tumor microenvironment have the ability to modulate CSC signalling pathway; however, their exact mechanism needs to be elucidated...
November 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Tulin Ozturk, Ozlem Kucukhuseyin, Allison Pinar Eronat, Mete Bora Tuzuner, Aynur Daglar-Aday, Neslihan Saygili, Halil Ibrahim Kisakesen, Fatih Seyhan, Mehmet Velidedeoğlu, Zerrin Calay, Şennur Ilvan, Hülya Yilmaz-Aydoğan, Oguz Ozturk, Turgar Isbir
miRNA involvement has been observed in almost every type of cancer, including breast cancer. The etiology of abnormal expression of miRNAs in cancer is still not clearly understood. In order to obtain insight into miRNA de-regulation in breast cancer, we analyzed expression levels of five breast cancer-related miRNAs, miRNA21, miRNA155, miRNA19a, miRNA17-5p and let7a miRNA, in both malignant and neighboring non-tumoral paraffin-embedded tissues of 47 patients with invasive ductal breast cancer. The targeted miRNAs, and a reference snRNA, U6, were analyzed by real-time polymerase chain reaction...
October 2015: Anticancer Research
J Z Gao, Y L Wang, J Li, L X Wei
The aim of this study was to investigate the effects of the vascular endothelial growth factor (VEGF)/VEGF receptor (VEGFR)/K-ras signaling pathways on miRNA21 levels in hepatocellular carcinoma tissues in rats. Eighteen male Sprague-Dawley rats were randomly divided into normal control, model, and VEGF blocking agent groups (N = 6/group). The expression of VEGF mRNA, K-ras protein, and miRNA21 increased significantly (P < 0.05) in the model group compared with the normal control group, and decreased dramatically in the VEGF blocking agent group compared to the model group...
January 30, 2015: Genetics and Molecular Research: GMR
Dongbei Li, Yinan Wang, Choiwan Lau, Jianzhong Lu
We have designed and evaluated novel stem-loop-structured probes for fluorescence detection of multiple microRNA (miRNA) targets. In the initial stage, the probes are in a closed stem conformation, shielding sterically a biotin label from being accessible to a fluorescence reporter. After hybridizing with target miRNAs, the probes undergo a conformational switch, restoring accessibility of the biotin to streptavidin-phycoerythin (SA-PE) for signal readout. Apparently, the bulky nature of the reporter SA-PE facilitates shielding of the biotin label in the absence of the target, thereby the stem-loop-structured probes allow sensitive detection of unlabeled miRNA targets, and xMAP array microspheres further realize simultaneous detection of multiple analytes using one fluorescence dye, SA-PE, for final readout...
October 21, 2014: Analytical Chemistry
Qingmin Wang, Shupeng Liu, Ying Tang, Qiuhong Liu, Yongjie Yao
MPT64 is one of the secreted proteins from Mycobacterium tuberculosis. Little is known about its role in infection by Mycobacterium tuberculosis. In this study, we demonstrated that MPT64 could dose-dependently inhibit the apoptosis of RAW264.7 macrophages induced by PPD-BCG. Quantitative real-time PCR results showed that the expression of bcl-2 increased in macrophages treated with MPT64 compared with PPD-treated cells. Furthermore, the results provided strong evidence that bcl-2 up-regulation was positively controlled by miRNA-21...
2014: PloS One
Liqun Chen, Lilly Y W Bourguignon
MicroRNA-21 (miR-21) is associated with the development of solid tumors progression including breast cancer. In this study we investigated matrix hyaluronan (HA)-CD44 (a primary HA receptor) interaction with c-Jun N-Terminal Kinase (JNK)/c-Jun signaling in MDA-MB-468 breast cancer cells [a triple-negative (estrogen receptor-negative/progesterone receptor-negative/HER2-negative) breast cancer cell line]. Our results indicated that HA binding to CD44 promotes c-Jun nuclear translocation and transcriptional activation...
2014: Molecular Cancer
O N Dreval', A S Belokhvostov, V M Feniksov
BACKGROUND: The purpose of this study is to assess clinical, morphofunctional and molecular factors of prognosis in patients with malignant cerebral gliomas. MATERIALS AND METHODS: Retrospective analysis of follow-up of surgical and complex treatment of 95 patients with cerebral gliomas was performed. The series included 81 primary case and 14 recurrent cases. In all patients age, sex, clinical, topographic, morphological and therapeutic factors were analyzed. In 47 patients molecular and genetic prognostic factors including p51, MGMT, p16, PTEN and hyperproduction of miRNA21 in tumor cells were investigated...
October 2009: Zhurnal Voprosy Neĭrokhirurgii Imeni N. N. Burdenko
M N Kagalwala, S K Singh, S Majumder
How the programming and reprogramming of stem/progenitor cells regulate normal cell development and cancer is still not well known. One of the tools that we have chosen to use to investigate stem cell regulation is the transcriptional repressor element 1-silencing transcription factor (REST). REST contains a DNA-binding domain and two repressor domains. Once bound to its target genes, REST can interact with several cellular corepressors to regulate epigenetic modifications. REST is expressed in most nonneural cells, including neural stem/progenitor cells (NSCs), but it is absent in most neuronal cells...
2008: Cold Spring Harbor Symposia on Quantitative Biology
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