Wei Li, David Baliu-Rodriguez, Sanduni H Premathilaka, Sharmila I Thenuwara, Jeffrey A Kimbrel, Ty J Samo, Christina Ramon, E Anders Kiledal, Sara R Rivera, Jenan Kharbush, Dragan Isailovic, Peter K Weber, Gregory J Dick, Xavier Mayali
Nutrient-induced blooms of the globally abundant freshwater toxic cyanobacterium Microcystis cause worldwide public and ecosystem health concerns. The response of Microcystis growth and toxin production to new and recycled nitrogen (N) inputs, and the impact of heterotrophic bacteria in the Microcystis phycosphere on these processes are not well understood. Here, using microbiome transplant experiments, cyanotoxin analysis, and nanometer-scale stable isotope probing to measure N incorporation and exchange at single cell resolution, we monitored the growth, cyanotoxin production, and microbiome community structure of several Microcystis strains grown on amino acids or proteins as the sole N source...
May 8, 2024: ISME Journal