K Suba, Y Patel, A Martin-Alonso, B Hansen, X Xu, A Roberts, M Norton, P Chung, J Shrewsbury, R Kwok, V Kalogianni, S Cheng, X Liu, K Kalyviotis, G A Rutter, B Jones, J Minnion, B M Owen, P Pantazis, W Distaso, D J Drucker, T M Tan, S R Bloom, K G Murphy, V Salem
BACKGROUND: Type 2 diabetes (T2D) is characterised by the loss of first-phase insulin secretion. We studied mice with β-cell selective loss of the glucagon receptor (Gcgr fl/fl X Ins-1Cre ), to investigate the role of intra-islet glucagon receptor signalling on pan-islet calcium activity and insulin secretion. METHODS: Metabolic profiling was conducted on Gcgr β-cell-/- and littermate controls. Crossing with GCaMP6f (STOP flox) animals further allowed for β-cell specific expression of a fluorescent calcium indicator...
April 25, 2024: Molecular Metabolism