Małgorzata Figiel, Filip Szubert, Enrico Luchinat, Piotr Bonarek, Anna Baranowska, Katarzyna Wajda-Nikiel, Mateusz Wilamowski, Piotr Miłek, Marta Dziedzicka-Wasylewska, Lucia Banci, Andrzej Górecki
Human protein Yin Yang 1 (YY1) controls the transcription of hundreds of genes both positively and negatively through interactions with a wide range of partner proteins. Results presented here from proteolytic sensitivity, calorimetry, circular dichroism, fluorescence, NMR, size-exclusion chromatography, SELEX, and EMSA show that purified YY1 forms dimers via its disordered N-terminal region with strong zinc-ion concentration dependence. The YY1 dimer is shown to bind tandem repeats of a canonical recognition DNA sequence with high affinity, and analysis of human YY1 regulatory sites shows that many contain repeats of its recognition elements...
December 26, 2022: Biochimica et Biophysica Acta. Gene Regulatory Mechanisms