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Denggao Huang, Hui Cao, Shunlan Wang, Linlin Zheng, Zongwen Chen, Xiaohong Wen, Shufang Zhang, Yang Xiang, Yuanhui Gao
To establish a method for isolation, culture and identification of adipose-derived mesenchymal stem cells (ASCs) from the inbreed line miniature pig of Wuzhishan (ILMW).
 Methods: A total of 100 g adipose tissues were obtained from subcutaneous tissues of neck in six-month old healthy ILMW (3 samples, male). ASCs from ILMW (ILMW-ASCs) were isolated from adipose tissues through 0.1% collagenase digestion. The cells at the 3rd, 5th, 8th, 13th passages were collected. Cell morphology, size, phenotype, cell cycle, and apoptosis were monitored...
March 28, 2019: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
Young-Hoon Jeong, Hye Min Oh, Man Ryul Lee, C-Yoon Kim, Chanyang Joo, Soon-Jung Park, Yun-Ho Song, Changhee Kang, Hyung-Min Chung, Sun-Woong Kang, Kang Moo Huh, Sung-Hwan Moon
Adipose-derived mesenchymal stem cells (AD-MSCs) have been studied as desirable cell sources for regenerative medicine and therapeutic application. However, it has still remained a challenge to obtain enough adequate and healthy cells in large quantities. To overcome this limitation, various biomaterials have been used to promote expansion of MSCs in vitro. Recently, hexanoyl glycol chitosan (HGC) was introduced as a new biomaterial for various biomedical applications, in particular 3D cell culture, because of its biodegradability, biocompatibility, and other promising biofunctional properties...
July 30, 2018: Polymers
Nicola Alessio, Tiziana Squillaro, Vincenzo Monda, Gianfranco Peluso, Marcellino Monda, Mariarosa Ab Melone, Umberto Galderisi, Giovanni Di Bernardo
BACKGROUND: Research on physiopathology of obesity may receive new hints from studies on skinny people (SP). These are individuals who show a poor or null gaining of body weight, in spite of high-calorie intake, by far exceeding the body requirements. AIM: To evaluate how circulating factors present in the SP sera may affect adipogenesis of mesenchymal stromal cells (MSCs). METHODS: We isolated MSCs from bone marrow of healthy donors with both normal body mass index (BMI) and caloric consumption...
March 26, 2019: World Journal of Stem Cells
Elena Zoico, Vanni Rizzatti, Gabriella Policastro, Maela Tebon, Elena Darra, Rossi Andrea Petronio, Gloria Mazzali, Francesco Fantin, Mauro Zamboni
Aging is a physiological process characterized by an age-progressive decline in intrinsic physiological functions, with an increased risk of developing chronic metabolic conditions, such as insulin resistance and diabetes. Furthermore, from a physiopathological point of view, several authors describe an association between oxidative stress, hypoxia and these metabolic conditions. It had been suggested that adipose tissue (AT) dysfunction, senescent cell accumulation and proinflammatory pathways may be involved in this processes...
March 27, 2019: Experimental Gerontology
Andrea C Postmus, Ines Sturmlechner, Johan W Jonker, Jan M van Deursen, Bart van de Sluis, Janine K Kruit
PURPOSE OF REVIEW: Senescent cells have recently been identified as key players in the development of metabolic dysfunction. In this review, we will highlight recent developments in this field and discuss the concept of targeting these cells to prevent or treat cardiometabolic diseases. RECENT FINDINGS: Evidence is accumulating that cellular senescence contributes to adipose tissue dysfunction, presumably through induction of low-grade inflammation and inhibition of adipogenic differentiation leading to insulin resistance and dyslipidaemia...
March 22, 2019: Current Opinion in Lipidology
Allyson K Palmer, Ming Xu, Yi Zhu, Tamar Pirtskhalava, Megan M Weivoda, Christine M Hachfeld, Larissa G Prata, Theo H van Dijk, Esther Verkade, Grace Casaclang-Verzosa, Kurt O Johnson, Harjunisa Cubro, Ewald J Doornebal, Mikolaj Ogrodnik, Diana Jurk, Michael D Jensen, Eduardo N Chini, Jordan D Miller, Aleksey Matveyenko, Michael B Stout, Marissa J Schafer, Thomas A White, LaTonya J Hickson, Marco Demaria, Vesna Garovic, Joseph Grande, Edgar A Arriaga, Folkert Kuipers, Thomas von Zglinicki, Nathan K LeBrasseur, Judith Campisi, Tamar Tchkonia, James L Kirkland
Adipose tissue inflammation and dysfunction are associated with obesity-related insulin resistance and diabetes, but mechanisms underlying this relationship are unclear. Although senescent cells accumulate in adipose tissue of obese humans and rodents, a direct pathogenic role for these cells in the development of diabetes remains to be demonstrated. Here, we show that reducing senescent cell burden in obese mice, either by activating drug-inducible "suicide" genes driven by the p16Ink4a promoter or by treatment with senolytic agents, alleviates metabolic and adipose tissue dysfunction...
March 25, 2019: Aging Cell
Elisabetta Manuela Foppiani, Olivia Candini, Ilenia Mastrolia, Alba Murgia, Giulia Grisendi, Anna Valeria Samarelli, Giulia Boscaini, Lucrezia Pacchioni, Massimo Pinelli, Giorgio De Santis, Edwin M Horwitz, Elena Veronesi, Massimo Dominici
BACKGROUND: The ex vivo expansion potential of mesenchymal stromal/stem cells (MSC) together with their differentiation and secretion properties makes these cells an attractive tool for transplantation and tissue engineering. Although the use of MSC is currently being tested in a growing number of clinical trials, it is still desirable to identify molecular markers that may help improve their performance both in vitro and after transplantation. METHODS: Recently, HOXB7 was identified as a master player driving the proliferation and differentiation of bone marrow mesenchymal progenitors...
March 19, 2019: Stem Cell Research & Therapy
Katarzyna Kornicka, Mohamad Al Naem, Michael Röcken, Marta Zmiertka, Krzysztof Marycz
Osteochondritis dissecans (OCD) in equids, especially in sport horses, has become a growing issue as it contributes to the occurrence of lameness. Thus the aim of this study was to investigate the cytophysiological properties of OCD chondrocytes including expression of chondrogenic genes, apoptosis, mitochondria dynamics and autophagy. Horse chondrocytes were isolated from healthy (HE) and OCD cartilages. Properties of cells were evaluated using multiple assays e.g., polymerase chain reaction (PCR), immunofluorescence, Western blot...
March 8, 2019: Journal of Clinical Medicine
Federica Facchin, Silvia Canaider, Eva Bianconi, Margherita Maioli, Umberto Santoro, Sara Santaniello, Valentina Basoli, Pier Mario Biava, Carlo Ventura
Human adult stem cells hold promise for regenerative medicine. They are usually expanded for multiple passages in vitro to increase cell yield prior to transplantation. Unfortunately, prolonged culture leads to cell senescence, a major drawback from successful outcomes in cell therapy approaches. Here, we show that an extract from early Zebrafish embryo (ZF1) counteracted senescence progression in human adipose-derived stem cells (hASCs) along multiple culture passages (from the 5th to the 20th). Exposure to ZF1 strongly reduced the expression of senescence marker beta-galactosidase...
March 1, 2019: Frontiers in Bioscience (Scholar Edition)
Meenakshi Gaur, Marek Dobke, Victoria V Lunyak
Human adipose-derived mesenchymal stem (stromal) cells (hADSC) represent an attractive source of the cells for numerous therapeutic applications in regenerative medicine. These cells are also an efficient model to study biological pathways of stem cell action, tissue injury and disease. Like any other primary somatic cells in culture, industrial-scale expansion of mesenchymal stromal cells (MSC) leads to the replicative exhaustion/senescence as defined by the "Hayflick limit." The senescence is not only greatly effecting in vivo potency of the stem cell cultures but also might be the cause and the source of clinical inconsistency arising from infused cell preparations...
March 6, 2019: Methods in Molecular Biology
Ruya Zhao, Rayan Kaakati, Xinjian Liu, Lingfan Xu, Andrew K Lee, Robin Bachelder, Chuan-Yuan Li, Scott T Hollenbeck
BACKGROUND: The tumor microenvironment within the breast is rich in adipose elements. The interaction between adipose cells and breast cancer is poorly understood, particularly as it pertains to patients with genetic susceptibility to breast cancer. This study focuses on the phenotype of human adipose-derived stem cells with the BRCA1 mutation and the effect they may have on breast cancer cell behavior. METHODS: CRISPR/Cas9 was used to generate de novo BRCA1-knockdown human adipose-derived stem cells...
March 2019: Plastic and Reconstructive Surgery
Tanya Debnath, Lakshmi Kiran Chelluri
Background: Mesenchymal stem cells have immense potential in stem cell-based therapies, however there is a pre-requisite to develop a curative cell dose. Adipose tissue-derived mesenchymal stem cells are promising mainly due to their potential abundance, immunomodulatory effect and remarkable differentiation potential. Nevertheless, senescence may develop during their in vitro expansion due to the incidence of genetic instability. Hence, it is important to attain an ideal balance between mesenchymal stem cell growth, quality and genetic integrity before their clinical use...
January 2019: Hematology, Transfusion and Cell Therapy
Pierre-Emmanuel Noly, Pauline Labbé, Nathalie Thorin-Trescases, Annik Fortier, Albert Nguyen, Eric Thorin, Michel Carrier
OBJECTIVES: A strong relationship between high circulating angiopoietin-like 2 (ANGPTL2) levels, a proinflammatory adipokine, and cardiovascular diseases has been reported. Our objective was to determine whether plasma ANGPTL2 and high-sensitivity C-reactive protein (hs-CRP) levels change postoperatively in patients who underwent heart valve surgery and/or coronary artery bypass grafting. We hypothesized that a corrective cardiac surgery would decrease ANGPTL2 levels. METHODS: In 47 prospectively recruited patients who underwent coronary artery bypass grafting (n = 16), valve replacement (n = 16), or both (n = 15), we measured plasma ANGPTL2 and hs-CRP levels preoperatively, at 24 hours, at 3 to 5 days (hospital discharge), and at 30 to 90 days (follow-up) after surgery...
January 8, 2019: Journal of Thoracic and Cardiovascular Surgery
Chul Kim, Ji-Min Park, Youngsook Song, Sunghoon Kim, Jisook Moon
Senescence in stem cells, which occurs as a consequence of chronic responses to the environment, defines the capacity of stem cells for proliferation and differentiation as well as their potential for tissue regeneration and homeostasis maintenance. Although stem cells reside under low oxygen pressure and the availability of oxygen is known to be a crucial determinant in their fate, the key modulators in stem cell aging and the underlying mechanism have yet to be unraveled. Human placenta-derived mesenchymal stem cells (hpMSCs) were cultured under hypoxia (3% O2 ) or normoxia (21% O2 ) to investigate the key factors that regulate stem cell senescence under hypoxic conditions...
January 31, 2019: Aging Cell
Luminita Labusca, Kaveh Mashayekhi
Cellular reprogramming and induced pluripotent stem cell (IPSC) technology demonstrated the plasticity of adult cell fate, opening a new era of cellular modelling and introducing a versatile therapeutic tool for regenerative medicine. While IPSCs are already involved in clinical trials for various regenerative purposes, critical questions concerning their medium- and long-term genetic and epigenetic stability still need to be answered. Pluripotent stem cells have been described in the last decades in various mammalian and human tissues (such as bone marrow, blood and adipose tissue)...
January 26, 2019: World Journal of Stem Cells
Kohsuke Shirakawa, Wataru Yano, Keisuke Inoue, Yoshinori Katsumata, Jin Endo, Motoaki Sano
We previously found that senescence of cluster of differentiation 4 (CD4) T cells is accelerated in the visceral adipose tissue (VAT) of mice with diet-induced obesity (DIO) due to a high-fat diet (HFD), and that these senescent-associated T cells cause chronic inflammation of visceral adipose tissue through secretion of osteopontin, provoking systemic insulin resistance. In this study, we examined whether the development of chronic inflammation and senescence-associated T cells in VAT of DIO mice was improved by long-term weight loss after switching to normal chow (NC) or by administration of a sodium glucose cotransporter 2 inhibitor (tofogliflozin)...
2019: PloS One
Maryam Musavi, Fatemeh Kohram, Mozhgan Abasi, Zohreh Bolandi, Mohammad Ajoudanian, Samira Mohammadi-Yeganeh, Seyed M Hashemi, Kazem Sharifi, Hamid Reza Fathi, Hossein Ghanbarian
Rn7SK is a conserved small nuclear noncoding RNA which its function in aging has not been studied. Recently, we have demonstrated that Rn7SK overexpression reduces cell viability and is significantly downregulated in stem cells, human tumor tissues, and cell lines. In this study, we analyzed the role of Rn7SK on senescence in adipose tissue-derived mesenchymal stem cells (AD-MSCs). For this purpose, Rn7SK expression was downregulated and upregulated via transfection and transduction, respectively, in AD-MSCs and subsequently, various distinct characteristics of senescence including cell viability, proliferation, colony formation, senescence-associated β galactosidase activity, and differentiation potency was analyzed...
January 13, 2019: Journal of Cellular Physiology
Atsuko Imai, Eka Fujimoto, Kaho Tamura, Masanori Utsuyama, Kazuto Sato
AIM: Maternal obesity and improper nutrition predispose offspring to chronic metabolic diseases. Although the frequency of these diseases increases with aging, the effect of a maternal high-fat diet on aged offspring remains elusive. MAIN METHODS: C57BL/6J female mice were fed a high-fat (HF) diet or a control (CON) diet and then mated. All offspring remained with their birth dam until weaning at 3 weeks. After weaning, the offspring from the HF and CON diet-fed dams were given either the HF diet or CON diet, which resulted in four groups: CON/CON, CON/HF, HF/CON, and HF/HF...
January 7, 2019: Life Sciences
Clara Lefranc, Malou Friederich-Persson, Laura Braud, Roberto Palacios-Ramirez, Susanne Karlsson, Nabiha Boujardine, Roberto Motterlini, Frederic Jaisser, Aurelie Nguyen Dinh Cat
Adipose tissue (AT) senescence and mitochondrial dysfunction are associated with obesity. Studies in obese patients and animals demonstrate that the MR (mineralocorticoid receptor) contributes to obesity-associated cardiovascular complications through its specific role in AT. However, underlying mechanisms remain unclear. This study aims to elucidate whether MR regulates mitochondrial function in obesity, resulting in AT premature aging and vascular dysfunction. Obese (db/db) and lean (db/+) mice were treated with an MR antagonist or a specific mitochondria-targeted antioxidant...
February 2019: Hypertension
Amiya Kumar Ghosh, Martin O'Brien, Theresa Mau, Nathan Qi, Raymond Yung
Visceral adipose tissue (VAT) inflammation plays a central role in longevity and multiple age-related disorders. Cellular Senescence (SEN) is a fundamental aging mechanism that contributes to age-related chronic inflammation and organ dysfunction, including VAT. Recent studies using heterochronic parabiosis models strongly suggested that circulating factors in young plasma alter the aging phenotypes of old animals. Our study investigated if young plasma rescued SEN phenotypes in the VAT of aging mice. With heterochronic parabiosis model using young (3 m) and old (18 m) mice, we found significant reduction in the levels pro-inflammatory cytokines and altered adipokine profile that are protective of SEN in the VAT of old mice...
December 26, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
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