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Annexin 2

Anna Kurpińska, Joanna Suraj, Emilia Bonar, Agnieszka Zakrzewska, Marta Stojak, Magdalena Sternak, Agnieszka Jasztal, Maria Walczak
INTRODUCTION: The tumor-promoting rearrangement of the lungs facilitates the process of cancer cell survival in a foreign microenvironment and enables their protection against immune defense. The study aimed to define the fingerprint of the early rearrangement of the lungs via the proteomic profiling of the lung tissue in the experimental model of tumor metastasis in a murine 4 T1 mammary adenocarcinoma. MATERIALS AND METHODS: The studies were performed on 7-8-week-old BALB/c female mice...
February 11, 2019: Experimental and Molecular Pathology
Alexandre D Gimenes, Bruna F D Andrade, José Victor P Pinotti, Sonia M Oliani, Orfa Y Galvis-Alonso, Cristiane D Gil
BACKGROUND: The inflammatory process has been described as a crucial mechanism in the pathophysiology of temporal lobe epilepsy. The anti-inflammatory protein annexin A1 (ANXA1) represents an interesting target in the regulation of neuroinflammation through the inhibition of leukocyte transmigration and the release of proinflammatory mediators. In this study, the role of the ANXA1-derived peptide Ac2-26 in an experimental model of status epilepticus (SE) was evaluated. METHODS: Male Wistar rats were divided into Naive, Sham, SE and SE+Ac2-26 groups, and SE was induced by intrahippocampal injection of pilocarpine...
February 12, 2019: Journal of Neuroinflammation
Azusa Ito, Satoru Tsuda, Hiroshi Kunikata, Asano Toshihumi, Kota Sato, Toru Nakazawa
The evaluation of retinal ganglion cell (RGC) death is a key part of retinal disease care. Previously, we used a Sytox Orange (SO)-based real-time imaging method to assess the RGCs in mice that underwent optic nerve crush. Here, we used N-methyl-D-aspartate (NMDA) injury in rats to confirm our model and assess the effect of neuroprotective agents on RGCs. The rats received NMDA injury and the intravitreal injection of SO, a cell-impermeant dyeing compound that targets nucleic acid. After ten minutes, non-invasive confocal scanning laser ophthalmoscopy visualized damaged or dying cells...
February 9, 2019: Brain Research
Katarzyna Magierowska, Dominik Bakalarz, Dagmara Wójcik, Anna Chmura, Magdalena Hubalewska-Mazgaj, Sabina Licholai, Edyta Korbut, Slawomir Kwiecien, Zbigniew Sliwowski, Grzegorz Ginter, Tomasz Brzozowski, Marcin Magierowski
BACKGROUND AND PURPOSE: Besides hydrogen sulfide (H2 S) and nitric oxide (NO), carbon monoxide (CO) contributes to the maintenance of gastric mucosal integrity. We investigated increased CO bioavailability effects on time-dependent dynamics of gastric ulcer healing mediated by particular growth factors, anti-inflammatory and molecular pathways. EXPERIMENTAL APPROACH: Wistar rats with gastric ulcers induced by serosal acetic acid application (day 0) were treated i...
February 9, 2019: Biochemical Pharmacology
Michael W Miller
BACKGROUND: Transforming growth factor (TGF) β1 and ethanol powerfully inhibit the proliferation, DNA repair, and survival of neural stem cells (NSCs). The present study tests the hypothesis that the ethanol-induced DNA damage response is mediated through p53 pathways and influenced by growth factor signals. METHODS: Cultures of non-immortalized NSCs, NS-5 cells, were transfected with p53 siRNA, exposed to either the mitogenic fibroblast growth factor (FGF) 2 or anti-mitogenic TGFβ1, and to ethanol...
February 12, 2019: Alcoholism, Clinical and Experimental Research
Yifeng Wang, Qingxin Song, Xuan Huang, Zhi Chen, Fan Zhang, Kun Wang, Guofeng Huang, Hongxing Shen
Nucleus pulposus cell (NPC) apoptosis serves an important role in intervertebral disc degeneration (IDD); however, the roles of long noncoding RNAs (lncRNAs) in this process remain unknown. The present study aimed to determine the effects of the lncRNA growth arrest‑specific transcript 5 (GAS5) on the apoptosis of primary human NPCs derived from the intervertebral disc, and to investigate the underlying mechanisms. TargetScan was used to predict the lncRNAs targeted by microRNA‑155 (miR‑155). Then, NPCs were subjected to lentivirus‑mediated transduction of miR‑155 or GAS5...
January 22, 2019: Molecular Medicine Reports
David Lagier, David Tonon, Philippe Garrigue, Benjamin Guillet, Laura Giacomino, Jean-Charles Martin, Marie-Christine Alessi, Nicolas Bruder, Lionel J Velly
BACKGROUND: Several lipid metabolites in cerebrospinal fluid are correlated with poor outcomes in aneurysmal subarachnoid haemorrhage. Most of these metabolites bind to ubiquitous thromboxane-prostaglandin (TP) receptors, causing vasoconstriction and inflammation. Here, we evaluated terutroban (TBN), a specific TP receptor antagonist, for the prevention of post-haemorrhage blood-brain barrier disruption, neuronal apoptosis and delayed cerebral hypoperfusion. METHODS: The rat double subarachnoid haemorrhage model was produced by twice injecting (days 1 and 2) autologous blood into the cisterna magna...
February 11, 2019: Critical Care: the Official Journal of the Critical Care Forum
Xi Zhang, Yuliang Pan, Huiqun Fu, Juan Zhang
BACKGROUND The inhibitor of apoptosis, B-cell lymphoma 2 (Bcl-2), is encoded by the BCL2 gene. Previous studies have shown that microRNAs are downregulated in prostate cancer. This study aimed to investigate the role of microRNA-205 and microRNA-338-3p and cell apoptosis in prostate carcinoma tissue and the LNCaP human prostate adenocarcinoma cell line by directly targeting the BCL2 gene and Bcl-2 protein expression. MATERIAL AND METHODS Bioinformatics methods predicted the target genes of miR-205 and miR-338-3p, which were validated by a luciferase assay...
February 11, 2019: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Na Li, Feng Yao, Huifang Huang, Hong Zhang, Wan Zhang, Xiangyang Zou, Linlin Sui, Lin Hou
Annexins are highly conserved and ubiquitous in various somatic cell types. They are involved in membrane transport and a range of calcium-regulated activities on the cell membrane surface, including vesicular transport, membrane fusion in exocytosis, signal transduction, and formation of calcium channels. They also regulate inflammatory response, cell differentiation, and interaction between cytoskeletal proteins. In this study, for the first time, an ANX3 gene from Artemia sinica ( As-anx3) was cloned. The As-anx3 full-length complementary DNA comprises 1,024 bp and has a 948 bp open reading frame encoding a 315-amino-acid polypeptide with four ANX domains...
February 11, 2019: Molecular Reproduction and Development
Xiaolong Gu, Zhi Wang, Jian Gao, Dandan Han, Limei Zhang, Peng Chen, Guangjian Luo, Bo Han
Fluoride is very crucial for development of teeth and bones. Excessive fluoride, however, causes damage to teeth and bones resulting in serious public health problem. SIRT1 regulates physiological and pathological processes such as apoptosis and cell cycle. Although SIRT1 inhibits p53-mediated transactivation, how SIRT1 regulates p53 in fluorosis remains unclear. This study aims to investigate the involvement of SIRT1 in fluoride-induced cell cycle arrest and apoptosis in MC3T3-E1 cells and the underlying mechanism...
February 7, 2019: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Jingxiang Zhao, Bocong Xu, Gan Chen, Yuhua Zhang, Quan Wang, Lian Zhao, Hong Zhou
BACKGROUND: Cryopreservation in dimethyl sulfoxide and storage at -80 °C extends the shelf life of platelets to at least 2 years, allowing greater availability in rural and military areas. While cryopreserved platelets (CPPs) have been extensively characterized for coagulation and thrombin generation, reports on the mechanism of adverse reactions to CPPs transfusion are scarce. Here, we tested the hypothesis that CPPs facilitate phagocytosis by Kupffer cells and subsequently promote the inflammatory response in Kupffer cells...
February 8, 2019: Transfusion
Nerella Sridhar Goud, S Mahammad Ghouse, Jatoth Vishnu, Jakkula Pranay, Ravi Alvala, Venu Talla, Insaf A Qureshi, Mallika Alvala
BACKGROUND: Human Galectin-1, protein of lectin family showing affinity towards β-galactosides has emerged as critical regulator of tumor progression and metastasis, by modulating diverse biological events including homotypic cell aggregation, migration, apoptosis, angiogenesis and immune escape. Therefore, galectin-1 inhibitors might represent novel therapeutic agents for cancer. METHODS: A new series of heterocyclic imines linked coumarin-thiazole hybrids (6a-6r) has been synthesized and evaluated for their cytotoxic potential against a panel of six human cancer cell lines namely, lung (A549), prostate (DU-145), breast (MCF-7 & MDA-MB-231), colon (HCT-15 & HT-29) using MTT assay...
February 7, 2019: Anti-cancer Agents in Medicinal Chemistry
Asif Raza, Siddhartha Sankar Ghosh
Background: Escherichia coli cytosine deaminase (CD) converts 5-fluorocytosine (5-FC), a prodrug, into 5-fluorouracil (5-FU), a chemotherapeutic drug. However, the poor binding affinity of CD towards 5-FC as compared to the natural substrate cytosine, limits its application towards a successful suicide gene therapy. Although F186W mutant was developed to enhance the effect of wild-type CD, still scope for its improvement remains to further minimize the dose-dependent cytotoxicity of the drugs...
2019: Biochemistry Insights
Raniere Fagundes Melo-Silveira, Rony Lucas Silva Viana, Diego Araujo Sabry, Rodrigo Augusto da Silva, Daisy Machado, Ana Karina Lima Nascimento, Katia Castanho Scortecci, Carmen Veríssima Ferreira-Halder, Guilherme Lanzi Sassaki, Hugo Alexandre Oliveira Rocha
Xylan is one of the most abundant hemicellulose constituents in the plant kingdom. We extracted xylan from corn cobs (XCC) using ultrasound. The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5. XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%...
April 15, 2019: Carbohydrate Polymers
Vivianne S Velozo-Sá, Luciano R Pereira, Aliny P Lima, Francyelli Mello-Andrade, Manuela R M Rezende, Rebeca M Goveia, Wanessa C Pires, Monize M Silva, Katia M Oliveira, Antonio G Ferreira, Javier Ellena, Victor M Deflon, Cesar Koppe Grisolia, Alzir A Batista, Elisângela P Silveira-Lacerda
In this paper, four new ruthenium complexes, [Ru(N-S)(dppm)2]PF6 (1), [Ru(N-S)(dppe)2]PF6 (2), [Ru(N-S)2(dppp)] (3) and [Ru(N-S)2(PPh3)2] (4) [dppm = 1,1-bis(diphenylphosphino)methane, dppe = 1,2-bis(diphenylphosphino)ethane, dppp = 1,3-bis(diphenylphosphino)propane, PPh3 = triphenylphosphine and N-S = 2-mercaptopyrimidine anion] were synthesized and characterized using spectroscopy techniques, molar conductance, elemental analysis, electrochemical techniques and X-ray diffraction. The DNA binding studies were investigated using voltammetry and spectroscopy techniques...
February 6, 2019: Dalton Transactions: An International Journal of Inorganic Chemistry
L V Wangxia, Yang Fang, Yongjun Liu, Yazhen Zhao, Zhong Shi, Haijun Zhong
Background/Aims: This study aimed to explore the effect of circular RNA ARHGAP26 (circ-ARHGAP26) on cell proliferation and apoptosis in gastric cancer (GC) cell lines. Materials and Methods: Human GC cell lines including HGC-27, AGS, SGC-7901, BGC-823, NCI-N87 and human normal gastric mucosal cells GSE-1 were cultured. The circ-ARHGAP26 expression was determined by quantitative polymerase chain reaction assay. Blank inhibitor and circ-ARHGAP26 inhibitor plasmids were transfected into HGC-27 or AGS cells as NC (-) and circ-ARHGAP26(-) groups...
February 1, 2019: Saudi Journal of Gastroenterology: Official Journal of the Saudi Gastroenterology Association
Qing-Song Lin, Wei-Xiong Wang, Yuan-Xiang Lin, Zhang-Ya Lin, Liang-Hong Yu, Yin Kang, De-Zhi Kang
OBJECTIVEGlutamate excitotoxicity and neuronal apoptosis are suggested to contribute to early brain injury after subarachnoid hemorrhage (SAH). Annexin A7 (ANXA7) has been shown to regulate glutamate release. However, the role of ANXA7 in early brain injury after SAH has not been illustrated. In this study, we aimed to investigate the effect of ANXA7 knockdown in reducing the severity of early brain injury after SAH, and determine the underlying mechanisms.METHODSEndovascular perforation was performed to induce SAH in male Sprague-Dawley rats...
February 1, 2019: Journal of Neurosurgery
Safa Ben Mimouna, Tifenn Le Charpentier, Sophie Lebon, Juliette Van Steenwinckel, Imed Messaoudi, Pierre Gressens
The present study examined the involvement of zinc (Zn)-transporters (ZnT3) in cadmium (Cd)-induced alterations of Zn homeostasis in rat hippocampal neurons. We treated primary rat hippocampal neurons for 24 or 48 hr with various concentrations of CdCl2 (0, 0.5, 5, 10, 25, or 50 μM) and/or ZnCl 2 (0, 10, 30, 50, 70, or 90 μM), using normal neuronal medium as control. By The CellTiter 96 ® Aqueous One Solution Cell Proliferation Assay (MTS; Promega, Madison, WI) assay and immunohistochemistry for cell death markers, 10 and 25 μM of Cd were found to be noncytotoxic doses, and both 30 and 90 μM of Zn as the best concentrations for cell proliferation...
February 4, 2019: Journal of Cellular Physiology
Teresia Maina, Tracy Prysliak, Jose Perez-Casal
Bacterial pathogens have evolved to manipulate host cell death and survival pathways for their intracellular persistence. Understanding the ability of a bacterium to induce or inhibit cell death is essential for elucidating the disease pathogenesis and suggesting potential therapeutic options to manage the infection. In recent years, apoptosis inhibition by different bacteria has been suggested as a mechanism of survival by allowing the pathogen to replicate and disseminate in the host. Mycoplasma bovis has evolved mechanisms to invade and modulate apoptosis of bovine peripheral blood mononuclear cells (PBMC), red blood cells (RBCs), primary macrophages and monocytes...
February 2019: Veterinary Immunology and Immunopathology
Lei Yu, Min Chen, Rui Zhang, Ting Xu
BACKGROUND Pancreatic cancer has high incidence and low survival rates around the globe, mainly due to late diagnosis and unavailability of efficient chemotherapeutic agents. In the present study, the anticancer potential of glychionide-A was examined against PANC-1 pancreatic cancer cells. MATERIAL AND METHODS CellTiter-Glo Luminescent Cell Viability Assay Kits were used for assessment of cell viability. Electron microscopy and DAPI staining were used for the detection of apoptosis and autophagy, respectively...
February 3, 2019: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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