Olga V Morozova, Elizaveta R Pavlova, Dmitry V Bagrov, Nikolay A Barinov, Kirill A Prusakov, Elena I Isaeva, Victor V Podgorsky, Dmitry V Basmanov, Dmitry V Klinov
Purpose: To develop a general method for NP fabrication from various proteins with maintenance of biological activity. Methods: A novel general approach for producing protein nanoparticles (NP) by nanoprecipitation of the protein solutions in 1,1,1,3,3,3-hexafluoroisopropanol is described. Protein NP sizes and shapes were analyzed by dynamic light scattering, scanning electron and atomic force microscopy (SEM and AFM). Chemical composition of the NP was confirmed using ultraviolet (UV) spectroscopy, energy-dispersive X-ray spectroscopy (EDX) and circular dichroism (CD)...
2018: International Journal of Nanomedicine