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Chemosensitivity Test

Rup K Sainju, Deidre N Dragon, Harold B Winnike, Marcus B Nashelsky, Mark A Granner, Brian K Gehlbach, George B Richerson
OBJECTIVE: Severe periictal respiratory depression is thought to be linked to risk of sudden unexpected death in epilepsy (SUDEP) but its determinants are largely unknown. Interindividual differences in the interictal ventilatory response to CO2 (hypercapnic ventilatory response [HCVR] or central respiratory CO2 chemosensitivity) may identify patients who are at increased risk for severe periictal hypoventilation. HCVR has not been studied previously in patients with epilepsy; therefore we evaluated a method to measure it at bedside in an epilepsy monitoring unit (EMU) and examined its relationship to postictal hypercapnia following generalized convulsive seizures (GCSs)...
February 12, 2019: Epilepsia
Rositsa Mihaylova, Anife Ahmedova, Denitsa Momekova, Georgi Momekov, Nikolay Danchev
Since the introduction of cisplatin into clinical practice a few decades ago, the topic of metal-based drugs has expanded significantly. Recent examples emphasize on metallosupramolecules as an emerging class of compounds with diverse properties. They can trigger unique cellular events in malignant cells or serve as molecular hosts for various biologically active compounds, including anticancer agents. The anthracene-shelled M2 L4 coordination nanocapsules under research have already proved very high anticancer potency with remarkable selectivity and lack of cross-resistance...
February 8, 2019: Investigational New Drugs
Aneta Kaczor, Karolina Witek, Sabina Podlewska, Joanna Czekajewska, Annamaria Lubelska, Ewa Żesławska, Wojciech Nitek, Gniewomir Latacz, Sandrine Alibert, Jean-Marie Pagès, Elżbieta Karczewska, Katarzyna Kieć-Kononowicz, Jadwiga Handzlik
Searching for new chemosensitizers of bacterial multidrug resistance (MDR), chemical modifications of (Z)-5-(4-chlorobenzylidene)-2-(4-methylpiperazin-1-yl)-3H-imidazol-4(5H)-one ( 6 ) were performed. New compounds ( 7 ⁻ 17 ), with fused aromatic rings at position 5, were designed and synthesized. Crystallographic X-ray analysis proved that the final compounds ( 7 ⁻ 17 ) were substituted with tertiary amine-propyl moiety at position 3 and primary amine group at 2 due to intramolecular Dimroth rearrangement...
January 26, 2019: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Han-Hee Park, Seung-Won Choi, Gwang Jin Lee, Young-Dae Kim, Hyun-Jin Noh, Seung-Jae Oh, Iseul Yoo, Yu-Jin Ha, Gi-Bang Koo, Soon-Sun Hong, Sung Won Kwon, You-Sun Kim
Background: Ginseng is believed to have antitumor activity. Autophagy is largely a prosurvival cellular process that is activated in response to cellular stressors, including cytotoxic chemotherapy; therefore, agents that inhibit autophagy can be used as chemosensitizers in cancer treatment. We examined the ability of Korean Red Ginseng extract (RGE) to prevent autophagic flux and to make hepatocellular carcinoma (HCC) cells become more sensitive to doxorubicin. Methods: The cytotoxic effects of total RGE or its saponin fraction (RGS) on HCC cells were examined by the lactate dehydrogenase assay in a dose- or time-dependent manner...
January 2019: Journal of Ginseng Research
Emmanuel Veríssimo de Araújo, Keyth Guimarães, Marciane Magnani, Josiane Cruz, Hubert Vidal, Valdir Andrade Braga, José Luiz de Brito Alves
Hypertension and metabolic disorders evidenced in adults who have been exposed to nutritional insults during early life may be sex-dependent. We evaluated whether blood pressure (BP), cardiorespiratory control and metabolic parameters are affected in female offspring (FO) from dams fed a dyslipidaemic diet during pregnancy and lactation. FO was obtained from dams who received control (CTL) or dyslipidaemic diet (DLP) during pregnancy and lactation. The effects of a maternal dyslipidaemic diet on BP, cardiorespiratory control and biochemical parameters were assessed at 30 and 90 days of age...
January 16, 2019: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
Yongqing Cai, Jian Huang, Haiyan Xing, Bin Li, Ling Li, Xianfeng Wang, Dan Peng, Jianhong Chen
Background/purpose: The aim of this study was to investigate the role and mechanisms of the formyl peptide receptor (FPR) and the toll-like receptor 9 (TLR9) in hypoxia-induced chemoresistance of human ovarian cancer cells. Materials and methods: SKOV3 cells were exposed to hypoxia for 24 hours, the supernatant was collected to stimulate normoxia-cultured SKOV3, and the inhibition rate of cell growth was detected with CCK8 test. The agonist of TLR9 CpG ODN and the agonist of FPR fMLF were applied to investigate the chemosensitivity of SKOV3 cells to cisplatin...
2019: OncoTargets and Therapy
Qi Guo, Yanmei Liu, Jiayuan Zhao, Jing Wang, Yue Li, Yunqing Pang, Jian Chen, Jing Wang
Objective: The aim of this study was to investigate whether evodiamine (EVO) could potentiate the antitumor activity of gemcitabine (GEM) in tongue cancer cells and determine its potential underlying mechanisms. Materials and methods: Human Tca8113 and CAL-27 tongue squamous carcinoma cell lines were treated with EVO and GEM in different sequences and doses, after which cell proliferation was measured. Drug interactions were analyzed using the Chou-Talalay method with CompuSyn software...
2019: OncoTargets and Therapy
Leylah M Drusbosky, Robinson Vidva, Saji Gera, Anjanasree V Lakshminarayana, Vijayashree P Shyamasundar, Ashish Kumar Agrawal, Anay Talawdekar, Taher Abbasi, Shireen Vali, Cristina E Tognon, Stephen E Kurtz, Jeffrey W Tyner, Shannon K McWeeney, Brian J Druker, Christopher R Cogle
Despite advances in understanding the molecular pathogenesis of acute myeloid leukaemia (AML), overall survival rates remain low. The ability to predict treatment response based on individual cancer genomics using computational modeling will aid in the development of novel therapeutics and personalize care. Here, we used a combination of genomics, computational biology modeling (CBM), ex vivo chemosensitivity assay, and clinical data from 100 randomly selected patients in the Beat AML project to characterize AML sensitivity to a bromodomain (BRD) and extra-terminal (BET) inhibitor...
January 7, 2019: Leukemia Research
Ansu Kumar, Leylah M Drusbosky, Amy Meacham, Madeleine Turcotte, Priyanka Bhargav, Sumanth Vasista, Shahabuddin Usmani, Anusha Pampana, Kabya Basu, Anuj Tyagi, Deepak Lala, Swaminathan Rajagopalan, Shivgonda C Birajdar, Aftab Alam, Kunal Ghosh Roy, Taher Abbasi, Shireen Vali, Manju Sengar, Girish Chinnaswamy, Bijal D Shah, Christopher R Cogle
Early T-cell precursor acute lymphoblastic leukemia (ETP-ALL) is an aggressive hematological malignancy for which optimal therapeutic approaches are poorly characterized. Using computational biology modeling (CBM) in conjunction with genomic data from cell lines and individual patients, we generated disease-specific protein network maps that were used to identify unique characteristics associated with the mutational profiles of ETP-ALL compared to non-ETP-ALL (T-ALL) cases and simulated cellular responses to a digital library of FDA-approved and investigational agents...
January 7, 2019: Leukemia Research
Moustafa S Ali, Abdelkader A Metwally, Rania H Fahmy, Rihab Osman
Remarkable efforts are currently devoted to the area of nanodiamonds (NDs) research due to their superior properties viz: biocompatibility, minute size, inert core, and tunable surface chemistry. The use of NDs for the delivery of anticancer drugs has been at the forefront of NDs applications owing to their ability to increase chemosensitivity, sustain drug release, and minimize drug side effects. Accelerated steps towards the move of NDs from bench side to bedside have been recently witnessed. In this review, the effects of NDs production and purification techniques on NDs' final properties are discussed...
January 11, 2019: International Journal of Pharmaceutics
Liping Tan, Dongmei Mai, Bailin Zhang, Xiaobing Jiang, Jialiang Zhang, Ruihong Bai, Ying Ye, Mei Li, Ling Pan, Jiachun Su, Yanfen Zheng, Zexian Liu, Zhixiang Zuo, Qi Zhao, Xiaoxing Li, Xudong Huang, Jie Yang, Wen Tan, Jian Zheng, Dongxin Lin
BACKGROUND: Breast cancer is one of the most common malignancies and the major cause of cancer-related death in women. Although the importance of PIWI-interacting RNAs (piRNAs) in cancer has been increasingly recognized, few studies have been explored the functional mechanism of piRNAs in breast cancer development and progression. METHODS: We examined the top 20 highly expressed piRNAs based on the analysis of TCGA breast cancer data in two patient cohorts to test the roles of piRNAs in breast cancer...
January 12, 2019: Molecular Cancer
Eun Mi Lee, Dong Hyun Kim, Do Young Kim, Young Mi Seol, Young Jin Choi, Hyojeong Kim
PURPOSE: Soft tissue sarcoma (STS) is a rare and heterogeneous cancer with over 50 known subtypes. It is difficult to understand the role of adjuvant treatment in STS. We aimed to determine the benefits of adjuvant treatment for a rare STS subset: non- extremity STS with moderate chemosensitivity. MATERIALS AND METHODS: We reviewed medical records from Pusan National University Hospital and Kosin University Gospel Hospital, which had detailed pathological reports on patients diagnosed between 2006 and 2016...
December 2018: Radiation Oncology Journal
Marcelle Paula-Ribeiro, Indyanara C Ribeiro, Liliane C Aranda, Talita M Silva, Camila M Costa, Roberta P Ramos, Jaquelina S Ota-Arakaki, Sergio L Cravo, Luiz E Nery, Michael K Stickland, Bruno M Silva
KEY POINTS: Dysfunction of post-exercise cardiac autonomic control is associated with increased mortality risk in healthy adults and in patients with cardiorespiratory diseases. The afferent mechanisms that regulate the post-exercise cardiac autonomic control remain unclear. We found that afferent signals from carotid chemoreceptors restrain the post-exercise cardiac autonomic control in healthy adults and patients with pulmonary arterial hypertension (PAH). Patients with PAH had higher carotid chemoreflex sensitivity, and the magnitude of carotid chemoreceptors restraint of autonomic control was greater in patients with PAH as compared to healthy adults...
January 9, 2019: Journal of Physiology
Juan Li, Zhan Qi, Yuan-Ping Hu, Yu-Xiang Wang
Esophageal cancer is the eighth most common form of cancer worldwide, and esophageal squamous cell carcinoma (ESCC) is a major type of esophageal cancer that arises from epithelial cells of the esophagus. Local lymph node metastasis (LNM) is a typical sign of failure for ESCC clinical treatments, and a link has been established between LNM and the aberrant expression of specific biomarkers. In this review, we summarize what is known about nine factors significantly associated with LNM in ESCC patients: phosphatase and tensin homolog (PTEN), mucin 1, vascular endothelial growth factor-C, tumor necrosis factor alpha-induced protein 8 (TNFAIP8), Raf-1 kinase inhibitory protein, stathmin (STMN1), metastasis-associated protein 1, caveolin-1, and interferon-induced transmembrane protein 3...
January 7, 2019: Journal of International Medical Research
Hiroshi Yaegashi, Kouji Izumi, Suguru Kadomoto, Renato Naito, Tomoyuki Makino, Hiroaki Iwamoto, Takahiro Nohara, Kazuyoshi Shigehara, Yoshifumi Kadono, Atsushi Mizokami
BACKGROUND: The present study was conducted to investigate whether baseline serum carbohydrate antigen (CA) 19-9 predicts prognosis or treatment effect in patients with advanced urothelial carcinoma (UC). MATERIALS AND METHODS: We retrospectively analyzed data of patients diagnosed with locally advanced or metastatic or recurrent UC between April 2008 and November 2014. CA19-9 was determined using enzyme-linked immunosorbent assay (ELISA) and the relationship between CA19-9 and prognosis were analyzed...
January 2019: Anticancer Research
Lv-Xia Dai, Jing Yang, Jian-Min Liu, Sizhou Huang, Bao-Ning Wang, Hong Li, Jie Yang, Zhong-Yi Zhao, Kang Cao, Ming-Yuan Li
PURPOSE: The cross-reacting material 197 (CRM197) is a mutation of the diphtheria toxin. The protein of CRM197 was used successfully for the therapy of various tumors in the recent studies. In this study, the recombinant adenoviruses containing the CRM197gene(AdCRM197) were used to enhance the cellar toxicity of gemcitabine in human glioma U87, U251, and H4 cells. PROCEDURES: MTT assay and flow cytometric analysis were performed to test the apoptosis of the U87, U251 and H4 cells with the combined treatment of AdCRM197 plus gemcitabine...
December 26, 2018: Cancer Biotherapy & Radiopharmaceuticals
Miriam Pons, Georg Nagel, Yanira Zeyn, Mandy Beyer, Teresa Laguna, Tina Brachetti, Andreas Sellmer, Siavosh Mahboobi, Roland Conradi, Falk Butter, Oliver H Krämer
Experiments with cultured mammalian cells represent an in vitro alternative to animal experiments. Fetal calf serum (FCS) is the most commonly used media supplement worldwide. FCS contains a mixture of largely undefined growth factors and cytokines, which support cell proliferation. This undefined nature of FCS is a source of experimental variation, undesired immune responses, possible contaminations, and because of its way of production an ethical concern. Thus, alternative, defined, valid, and reliable media supplements should be characterized in a large number of experiments...
December 14, 2018: ALTEX
Faping You, Hong Luan, Dan Sun, Tao Cui, Pengpeng Ding, Haitao Tang, Diwen Sun
To explore the effect of miR-106a in breast cancer cell behavior and sensitivity to chemotherapeutic agents. Tumor tissue and adjacent normal tissue were derived from 40 breast cancer patients, and miR-106a expression was measured by reverse transcription-qPCR. Breast cancer cells, MDA-MB-231 and MCF-7, were treated with miRNA-106a mimic (MM) or miRNA-106a inhibitor (MI) and negative controls. Cell proliferation was measured by MTT assay. Clonogenicity was measured by colony-forming assay. Cell migration and invasion ability were measured by scratch test and transwell assay, respectively...
December 20, 2018: DNA and Cell Biology
Jaihwan Kim, Haeryoung Kim, Jong-Chan Lee, Jin Won Kim, Woo Hyun Paik, Sang Hyub Lee, Jin-Hyeok Hwang, Ji Kon Ryu, Yong-Tae Kim
Gemcitabine is a principal chemotherapeutic agent for biliary tract cancer (BTC). Expression of human equilibrative nucleoside transporter 1 (hENT1) is regarded as a potential predictive biomarker for a gemcitabine response in some cancers. This study was conducted to investigate the association between hENT1 expression and the effects of gemcitabine on BTC cell lines and on patients with advanced BTC receiving gemcitabine-based chemotherapy. A total of four BTC cell lines, HuCCT1, SNU-478, SNU-1079, and SNU-1196, were tested...
2018: PloS One
Fuxing Zhou, Xiaoshan Yang, Hang Zhao, Yu Liu, Yang Feng, Rui An, Xiaohui Lv, Jia Li, Biliang Chen
Cisplatin resistance significantly affects the survival rate of patients with ovarian cancer. However, the main mechanism underlying cisplatin resistance in ovarian cancer remains unclear. Methods: Immunohistochemistry was used to determine the expression of OGT, OGA and O -GlcNAc in chemoresistant and chemosensitive ovarian cancer tissues. Functional analyses (in vitro and in vivo) were performed to confirm the role of OGT in cisplatin resistance. Autophagy-related proteins were tested by western blot. Transmission electron microscopy and mRFP-GFP-LC3 adenovirus reporter were used for autophagy flux analysis...
2018: Theranostics
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