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https://read.qxmd.com/read/30772063/stable-force-control-and-contact-transition-of-a-single-link-flexible-robot-using-a-fractional-order-controller
#1
Daniel Feliu-Talegon, Vicente Feliu-Batlle, Inés Tejado, Blas M Vinagre, S Hassan HosseinNia
The control of robots that interact with the environment is an open area of research. Two applications that benefit from this study are: the control of the force exerted by a robot on an object, which allows the robot to perform complex tasks like assembly operations, and the control of collisions, which allows the robot safely collaborate with humans. Robot control is difficult in these cases because: (1) bouncing between free and constrained motion appears that may cause instability, (2) switching between free motion (position) controller and constrained motion (force) controller is required being the switching instants difficult to know and (3) robot control must be robust since the mechanical impedance of the environment is unknown...
February 5, 2019: ISA Transactions
https://read.qxmd.com/read/30771980/a-novel-fluorescence-turn-off-on-sensor-based-on-n-doped-graphene-quantum-dots-in-amino-acid-ionic-liquid-medium-and-its-application
#2
Wei Liu, Xiashi Zhu
The spectrum behavior of N-doped graphene quantum dots (N-GQD) in 1-hexyl-3-methylimidazolium tryptophan ionic liquid (TryIL) was studied. It was showed that TryIL could self-aggregate into water/oil micellar in aqueous solution and N-GQD was assembled on hydrophilic part of TryIL micelle to construct a novel fluorescence "turn on-off" probe (N-GQD-TryIL). the fluorescence intensity was quenched through the formation of N-GQD-TryIL-Cu(II) complex due to the strong binding ability between Cu2+ and N-GQD-TryIL...
May 15, 2019: Talanta
https://read.qxmd.com/read/30771487/fmrpolyg-alters-mitochondrial-transcripts-level-and-respiratory-chain-complex-assembly-in-fragile-x-associated-tremor-ataxia-syndrome-fxtas
#3
Dhruv Gohel, Lakshmi Sripada, Paresh Prajapati, Kritarth Singh, Milton Roy, Darshan Kotadia, Flora Tassone, Nicolas Charlet-Berguerand, Rajesh Singh
Fragile X-associated tremor/ataxia syndrome (FXTAS) is an inherited neurodegenerative disorder caused by an expansion of 55 to 200 CGG repeats (premutation) in FMR1. These CGG repeats are Repeat Associated non-ATG (RAN) translated into a small and pathogenic protein, FMRpolyG. The cellular and molecular mechanisms of FMRpolyG toxicity are unclear. Various mitochondrial dysfunctions have been observed in FXTAS patients and animal models. However, the causes of these mitochondrial alterations are not well understood...
February 13, 2019: Biochimica et biophysica acta. Molecular basis of disease
https://read.qxmd.com/read/30771468/self-association-of-cyclodextrins-and-cyclodextrin-complexes-in-aqueous-solutions
#4
Thorsteinn Loftsson, Phennapha Saokham, André Rodrigues Sá Couto
Cyclodextrins (CDs) are oligosaccharides that self-assemble in aqueous solutions to form transient clusters, nanoparticles and small microparticles. The critical aggregation concentration (cac) of the natural αCD, βCD and γCD in pure aqueous solutions was estimated to be 25, 8 and 9 mg/ml, respectively. The cac of 2-hydroxypropyl-β-cyclodextrin (HPβCD), that consists of mixture of isomers, was estimated to be significantly higher or 118 mg/ml. Addition of chaotropic agents (i.e. that disrupts non-covalent bonds such as hydrogen bonds) to the aqueous media increases the cac...
February 13, 2019: International Journal of Pharmaceutics
https://read.qxmd.com/read/30771202/a-new-twist-on-tropomyosin-binding-to-actin-filaments-perspectives-on-thin-filament-function-assembly-and-biomechanics
#5
William Lehman, Michael J Rynkiewicz, Jeffrey R Moore
Tropomyosin, best known for its role in the steric regulation of muscle contraction, polymerizes head-to-tail to form cables localized along the length of both muscle and non-muscle actin-based thin filaments. In skeletal and cardiac muscles, tropomyosin, under the control of troponin and myosin, moves in a cooperative manner between blocked, closed and open positions on filaments, thereby masking and exposing actin-binding sites necessary for myosin crossbridge head interactions. While the coiled-coil signature of tropomyosin appears to be simple, closer inspection reveals surprising structural complexity required to perform its role in steric regulation...
February 15, 2019: Journal of Muscle Research and Cell Motility
https://read.qxmd.com/read/30771045/comparative-transcriptomics-analysis-uncovers-alternative-splicing-events-and-molecular-markers-in-cabbage-brassica-oleracea-l
#6
Yuanyuan Xu, Aisong Zeng, Lixiao Song, Jiaqing Li, Jiyong Yan
Alternative splicing (AS) events were identified and verified in cabbage by comparative transcriptome analysis. The corresponding markers were developed and the germplasm resources were identified. Alternative splicing (AS) is a central regulatory mechanism that greatly contributes to plant gene expression and transcriptome diversity. A large body of evidence has shown that AS complexity is relevant for plant development, evolution, complexity, and adaptation. Both insertion/deletion (InDel) and single nucleotide polymorphism (SNP) are typically co-dominant inheritance markers and have abundant polymorphisms...
February 15, 2019: Planta
https://read.qxmd.com/read/30770943/comparative-genomics-siderophore-production-and-iron-scavenging-potential-of-root-zone-soil-bacteria-isolated-from-concord-grape-vineyards
#7
Ricky W Lewis, Anjuman Islam, Lee Opdahl, Joan R Davenport, Tarah S Sullivan
Iron (Fe) deficiency in crop production is a worldwide problem which often results in chlorosis in grapevines, particularly in calcareous soils. Siderophores secreted by microorganisms and Strategy II plants can chelate Fe and other metals in soil solution, and siderophore-Fe complexes can then be utilized by plants and microbes. Plants may also shift rhizosphere conditions to favor siderophore-producing microbes, which can increase plant available Fe. Between-row cover crops (barley, rye, wheat, wheat/vetch) were planted as living mulch to address grapevine chlorosis by enhancing soil health in two vineyards in central Washington...
February 15, 2019: Microbial Ecology
https://read.qxmd.com/read/30770899/resistance-to-anti-microtubule-drug-induced-cell-death-is-determined-by-regulation-of-bimel-expression
#8
Weimei Ruan, Gireedhar Venkatachalam, Radoslaw Mikolaj Sobota, Liyan Chen, Loo Chien Wang, Alena Jacobson, Kathirvel Paramasivam, Uttam Surana
Anti-microtubule agents are frequently used as anticancer therapeutics. Cell death induced by these agents is considered to be due to sustained mitotic arrest caused by the activation of spindle assembly checkpoint (SAC). However, some cell types are resistant to mitotic cell death. Cells' ability to escape mitotic arrest (mitotic slippage) is thought to be a major mechanism contributing to this resistance. Here, we show that resistance to cell death induced by anti-mitotic agents is not linked to cells' capacity to undergo mitotic slippage as generally believed but is dependent on the state of BimEL regulation during mitosis...
February 15, 2019: Oncogene
https://read.qxmd.com/read/30770847/mapb-the-brucella-suis-tamb-homologue-is-involved-in-cell-envelope-biogenesis-cell-division-and-virulence
#9
Magalí Graciela Bialer, Verónica Ruiz-Ranwez, Gabriela Sycz, Silvia Marcela Estein, Daniela Marta Russo, Silvia Altabe, Rodrigo Sieira, Angeles Zorreguieta
Brucella species are Gram-negative, facultative intracellular pathogens responsible for a worldwide zoonosis. The envelope of Brucella exhibits unique characteristics that make these bacteria furtive pathogens and resistant to several host defence compounds. We have identified a Brucella suis gene (mapB) that appeared to be crucial for cell envelope integrity. Indeed, the typical resistance of Brucella to both lysozyme and the cationic lipopeptide polymyxin B was markedly reduced in a ∆mapB mutant. MapB turned out to represent a TamB orthologue...
February 15, 2019: Scientific Reports
https://read.qxmd.com/read/30770712/development-and-validation-of-the-european-quality-equal-score-for-mucormycosis-management-in-haematology
#10
Philipp Koehler, Sibylle C Mellinghoff, Katrien Lagrou, Alexandre Alanio, Dorothee Arenz, Martin Hoenigl, Felix C Koehler, Cornelia Lass-Flörl, Jacques F Meis, Malcolm Richardson, Oliver A Cornely
BACKGROUND: Mucormycosis is a life-threatening infection in immunocompromised patients and in haematological malignancy patients in particular. OBJECTIVES: Our aim was to develop and evaluate a scoring tool to measure adherence to current guidelines for mucormycosis. METHODS: Current guidelines of scientific societies on mucormycosis management were reviewed. We assembled diagnostic, treatment and follow-up milestones and designed the EQUAL Mucormycosis Score...
February 15, 2019: Journal of Antimicrobial Chemotherapy
https://read.qxmd.com/read/30770648/impact-of-modified-gelatin-on-valvular-microtissues
#11
A Roosens, Y Handoyo, P Dubruel, H Declercq
A significant challenge in the field of tissue engineering is the biofabrication of three-dimensional (3D) functional tissues with direct applications in organ-on-a-chip systems and future organ engineering. Multi-cellular valvular microtissues can be used as building blocks for the formation of larger-scale valvular macrotissues. Yet, for the controlled biofabrication of 3D macrotissues with predefined complex shapes, directed assembly of microtissues through bioprinting is needed. This study aimed to investigate if modified gelatin is an instructive material for valvular microtissues...
February 15, 2019: Journal of Tissue Engineering and Regenerative Medicine
https://read.qxmd.com/read/30770470/hdac6-regulates-dna-damage-response-via-deacetylating-mlh1
#12
Mu Zhang, Chen Hu, Niko Moses, Joshua Haakenson, Shengyan Xiang, Daniel Quan, Bin Fang, Zhe Yang, Wenlong Bai, Gerold Bepler, Guo-Min Li, Xiaohong Mary Zhang
MLH1 is a key DNA mismatch repair (MMR) protein, which plays an important role in maintenance of genomic stability and the DNA damage response (DDR). Here, we report that MLH1 is a novel substrate of HDAC6. HDAC6 interacts with and deacetylates MLH1 both in vitro and in vivo. Interestingly, deacetylation of MLH1 blocks the assembly of the MutSα-MutLa complex. Moreover, we have identified four novel acetylation sites in MLH1 by mass spectrometry analysis. The deacetylation mimetic mutant, but not the wild-type and the acetylation mimetic mutant, of MLH1 confers resistance to 6-thioguanine...
February 15, 2019: Journal of Biological Chemistry
https://read.qxmd.com/read/30770433/cyclin-b3-is-required-for-metaphase-to-anaphase-transition-in-oocyte-meiosis-i
#13
Yufei Li, Leyun Wang, Linlin Zhang, Zhengquan He, Guihai Feng, Hao Sun, Jiaqiang Wang, Zhikun Li, Chao Liu, Jiabao Han, Junjie Mao, Pengcheng Li, Xuewei Yuan, Liyuan Jiang, Ying Zhang, Qi Zhou, Wei Li
Meiosis with a single round of DNA replication and two successive rounds of chromosome segregation requires specific cyclins associated with cyclin-dependent kinases (CDKs) to ensure its fidelity. But how cyclins control the distinctive meiosis is still largely unknown. In this study, we explored the role of cyclin B3 in female meiosis by generating Ccnb3 mutant mice via CRISPR/Cas9. Ccnb3 mutant oocytes characteristically arrested at metaphase I (MetI) with normal spindle assembly and lacked enough anaphase-promoting complex/cyclosome (APC/C) activity, which is spindle assembly checkpoint (SAC) independent, to initiate anaphase I (AnaI)...
February 15, 2019: Journal of Cell Biology
https://read.qxmd.com/read/30769920/tho-complex-subunit-7-homolog-negatively-regulates-cellular-antiviral-response-against-rna-viruses-by-targeting-tbk1
#14
Tian-Sheng He, Tao Xie, Jing Li, Ya-Xian Yang, Changsheng Li, Weiying Wang, Lingzhen Cao, Hua Rao, Cynthia Ju, Liang-Guo Xu
RNA virus invasion induces a cytosolic RIG-I-like receptor (RLR) signaling pathway by promoting assembly of the Mitochondrial antiviral-signaling protein (MAVS) signalosome and triggers the rapid production of type I interferons (IFNs) and proinflammatory cytokines. During this process, the pivotal kinase TANK binding kinase 1 (TBK1) is recruited to the MAVS signalosome to transduce a robust innate antiviral immune response by phosphorylating transcription factors interferon regulatory factor 3 (IRF3) and nuclear factor (NF)-κB and promoting their nuclear translocation...
February 15, 2019: Viruses
https://read.qxmd.com/read/30769894/cilia-distal-domain-diversity-in-evolutionarily-conserved-structures
#15
REVIEW
Helena Soares, Bruno Carmona, Sofia Nolasco, Luís Viseu Melo, João Gonçalves
Eukaryotic cilia are microtubule-based organelles that protrude from the cell surface to fulfill sensory and motility functions. Their basic structure consists of an axoneme templated by a centriole/basal body. Striking differences in ciliary ultra-structures can be found at the ciliary base, the axoneme and the tip, not only throughout the eukaryotic tree of life, but within a single organism. Defects in cilia biogenesis and function are at the origin of human ciliopathies. This structural/functional diversity and its relationship with the etiology of these diseases is poorly understood...
February 14, 2019: Cells
https://read.qxmd.com/read/30769859/ammonia-responsive-luminescence-of-ln-3-%C3%AE-diketonate-complex-encapsulated-within-zeolite-y
#16
Yuchen Deng, Peng Li, Yige Wang, Tianren Wang, Huanrong Li
Assembling Ln3+ (HPBAn ) (Ln = Eu or Tb, HPBA = N-(2-pyridinyl)benzoylacetamide) in the cavities of zeolite Y (ZY) via the "ship-in-a-bottle" strategy leads to the formation of novel luminescent composite, Ln(HPBAn )@ZY, whose luminescence can be easily modulated by ammonia on the basis of the energy level variation of HPBA after deprotonation process. Additionally the bimetallic complex doping sample, Eu0.5 Tb0.5 (HPBAn )@ZY, show great potential as self-referencing luminescent sensor for detecting low ammonia concentration of 10-12 ⁻0...
February 14, 2019: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/30769331/excel-combining-u-ex-u-trusion-printing-on-u-ce-u-llulose-scaffolds-with-u-l-u-amination-to-create-i-in-vitro-i-biological-models
#17
Alireza Shahin-Shamsabadi, P Ravi Selvaganapathy
Bioprinting is rapidly developing to be a powerful tool in tissue engineering for both whole tissue printing and development of in vitro models that can be used in drug discovery, toxicology and as in vitro bioreactors. Nevertheless, the ability to create complex 3D culture systems with different types of cells, extracellular matrices integrated with perfusable channels has been a challenge. Here we develop an approach that combines xurography of a scaffold material (cellulose) with extrusion printing of bioinks on it, followed by assembly in a layer by layer fashion to create complex 3D culture systems that could be used as in vitro models of biological processes...
February 15, 2019: Biofabrication
https://read.qxmd.com/read/30769160/a-thermodynamically-constrained-mathematical-model-for-the-kinetics-and-regulation-of-nadph-oxidase-2-complex-mediated-electron-transfer-and-superoxide-production
#18
Namrata Tomar, Shima Sadri, Allen W Cowley, Chun Yang, Nabeel Quryshi, Venkat R Pannala, Said H Audi, Ranjan K Dash
Reactive oxygen species (ROS) play an important role in cell signaling, growth, and immunity. However, when produced in excess, they are toxic to the cell and lead to premature aging and a myriad of pathologies, including cardiovascular and renal diseases. A major source of ROS in many cells is the family of NADPH oxidase (NOX), comprising of membrane and cytosolic components. NOX2 is among the most widely expressed and well-studied NOX isoform. Although details on the NOX2 structure, its assembly and activation, and ROS production are well elucidated experimentally, there is a lack of a quantitative and integrative understanding of the kinetics of NOX2 complex, and the various factors such as pH, inhibitory drugs, and temperature that regulate the activity of this oxidase...
February 12, 2019: Free Radical Biology & Medicine
https://read.qxmd.com/read/30768924/methodological-aspects-of-universal-immuno-pcr-on-standard-tubes
#19
J E Abud, C G Santamaría, M Oggero, H A Rodriguez
One of the most used formats in inmuno-polymerase chain reaction (IPCR) is known as "Universal" IPCR (signal-generating complexes is based on conjugates of biotinylated DNA, biotinylated IgG and avidin). In the present study, we evaluated the utility of using mono- and bi-biotinylated DNA probes, pre-self-assembled DNA-neutravidin complex, blocking step and glutaraldehyde pretreatment of standard PCR tubes to improve the analytical performance of the hTSH-IPCR assay. The use of pre-self-assembled mono-biotinylated DNA-neutravidin complex enhances both the sensitivity and the reproducibility of the hTSH-IPCR assay, even without blocking step: hTSH-IPCR assay showed an improved limit of detection (LOD: 0...
February 12, 2019: Analytical Biochemistry
https://read.qxmd.com/read/30768805/modeling-large-protein-glycosaminoglycan-complexes-using-a-fragment-based-approach
#20
Sergey A Samsonov, Martin Zacharias, Isaure Chauvot de Beauchene
Glycosaminoglycans (GAGs), a major constituent of the extracellular matrix, participate in cell-signaling by binding specific proteins. Structural data on protein-GAG interactions are crucial to understand and modulate these signaling processes, with potential applications in regenerative medicine. However, experimental and theoretical approaches used to study GAG-protein systems are challenged by GAGs high flexibility limiting the conformational sampling above a certain size, and by the scarcity of GAG-specific docking tools compared to protein-protein or protein-drug docking approaches...
February 15, 2019: Journal of Computational Chemistry
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