Victoria R Litvinova, Andrey P Rudometov, Nadezhda B Rudometova, Denis N Kisakov, Mariya B Borgoyakova, Lyubov A Kisakova, Ekaterina V Starostina, Anastasia A Fando, Vladimir A Yakovlev, Elena V Tigeeva, Ksenia I Ivanova, Andrei S Gudymo, Tatiana N Ilyicheva, Vasiliy Yu Marchenko, Artemiy A Sergeev, Alexander A Ilyichev, Larisa I Karpenko
The development of a safe and effective vaccine against avian influenza A virus (AIV) H5N8 is relevant due to the widespread distribution of this virus in the bird population and the existing potential risk of human infection, which can lead to significant public health concerns. Here, we developed an experimental pVAX-H5 DNA vaccine encoding a modified trimer of AIV H5N8 hemagglutinin. Immunization of BALB/c mice with pVAX-H5 using jet injection elicited high titer antibody response (the average titer in ELISA was 1 × 105 ), and generated a high level of neutralizing antibodies against H5N8 and T-cell response, as determined by ELISpot analysis...
May 14, 2024: Vaccines