Roland Immler, Katrin Nussbaumer, Axel Doerner, Omar El Bounkari, Silke Huber, Janine Abisch, Matteo Napoli, Sarah Schmidt, Andreas Margraf, Monika Pruenster, Ina Rohwedder, Baerbel Lange-Sperandio, Marcus A Mall, Renske de Jong, Caspar Ohnmacht, Juergen Bernhagen, David Voehringer, Jamey D Marth, David Frommhold, Markus Sperandio
Eosinophil recruitment is a pathological hallmark of many allergic and helminthic diseases. Here, we investigated chemokine receptor CCR3-induced eosinophil recruitment in sialyltransferase St3gal4-/- mice. We found a marked decrease in eosinophil extravasation into CCL11-stimulated cremaster muscles and into the inflamed peritoneal cavity of St3gal4-/- mice. Ex vivo flow chamber assays uncovered reduced adhesion of St3gal4 -/- compared to wild type eosinophils. Using flow cytometry, we show reduced binding of CCL11 to St3gal4-/- eosinophils...
May 7, 2024: Proceedings of the National Academy of Sciences of the United States of America