Ewelina Palak, Weronika Lebiedzinska, Oana Lupu, Kamila Pulawska, Slawomir Anisimowicz, Aleksandra N Mieczkowska, Maria Sztachelska, Gabriella N Niklinska, Gabriela Milewska, Monika Lukasiewicz, Donata Ponikwicka-Tyszko, Ilpo Huhtaniemi, Slawomir Wolczynski
Bisphenol A (BPA) exposure may impair gonadal steroidogenesis, although the underlying mechanism is not well known. Hereby, we assessed BPA action on human primary granulosa (hGC) and mouse Leydig cells (BLTK-1) proliferation, cytotoxicity, hormone secretion, and steroidogenic enzyme/receptor gene profile. hGC and BLTK-1 cells were stimulated with increasing concentrations of BPA (10-12 M to 10-4 M for cell proliferation assay, 10-8 M to 10-4 M for LDH-cytotoxicity assay, and 10-9 M to 10-5 M for hormone secretion and genes expression analysis)...
April 19, 2023: Reproductive Biology