K Araki-Sasaki, Y Ohashi, T Sasabe, K Hayashi, H Watanabe, Y Tano, H Handa
PURPOSE: The authors attempted to immortalize human corneal epithelial cells; it is difficult to propagate primary human corneal epithelial cells because of scarcity of available tissue. However, cell immortalization by virus is always accompanied by shedding of free virus. The current study was performed to establish a cell line that produces no free viral particle. METHODS: Primary cultured human corneal epithelial cells were infected with a recombinant sv40-adenovirus vector and were cloned three times to obtain a continuously growing cell line...
March 1995: Investigative Ophthalmology & Visual Science