Renata Mangione, Cesarina Giallongo, Andrea Duminuco, Enrico La Spina, Lucia Longhitano, Sebastiano Giallongo, Daniele Tibullo, Giuseppe Lazzarino, Miriam Wissam Saab, Arianna Sbriglione, Giuseppe A Palumbo, Andrea Graziani, Amer M Alanazi, Valentina Di Pietro, Barbara Tavazzi, Angela Maria Amorini, Giacomo Lazzarino
To date, little is known concerning the circulating levels of biochemically relevant metabolites (antioxidants, oxidative/nitrosative stress biomarkers, purines, and pyrimidines) in patients with primary myelofibrosis (PMF), a rare form of myeloproliferative tumor causing a dramatic decrease in erythropoiesis and angiogenesis. In this study, using a targeted metabolomic approach, serum samples of 22 PMF patients and of 22 control healthy donors were analyzed to quantify the circulating concentrations of hypoxanthine, xanthine, uric acid (as representative purines), uracil, β-pseudouridine, uridine (as representative pyrimidines), reduced glutathione (GSH), ascorbic acid (as two of the main water-soluble antioxidants), malondialdehyde, nitrite, nitrate (as oxidative/nitrosative stress biomarkers) and creatinine, using well-established HPLC method for their determination...
April 19, 2024: Antioxidants (Basel, Switzerland)