Kun Yin, Meijuan Zhao, Yiling Xu, Zhong Zheng, Shanqing Huang, Dianyi Liang, He Dong, Ye Guo, Li Lin, Jia Song, Huimin Zhang, Junhua Zheng, Zhi Zhu, Chaoyong Yang
Single-cell RNA sequencing (scRNA-seq) is a transformative technology that unravels the intricate cellular state heterogeneity. However, the Poisson-dependent cell capture and low sensitivity in scRNA-seq methods pose challenges for throughput and samples with a low RNA-content. Herein, to address these challenges, we present Well-Paired-Seq2 (WPS2), harnessing size-exclusion and quasi-static hydrodynamics for efficient cell capture. WPS2 exploits molecular crowding effect, tailing activity enhancement in reverse transcription, and homogeneous enzymatic reaction in the initial bead-based amplification to achieve 3116 genes and 8447 transcripts with an average of ∼20000 reads per cell...
April 10, 2024: Analytical Chemistry