keyword
https://read.qxmd.com/read/38739855/cryoprotective-polysaccharides-with-ordered-gel-structures-induce-ice-growth-anticipation-and-survival-enhancement-during-cell-cryopreservation
#1
JOURNAL ARTICLE
Bruno M Guerreiro, M Madalena Dionísio, João Carlos Lima, Jorge Carvalho Silva, Filomena Freitas
This work cross-correlated rheological, thermodynamic, and conformational features of several natural polysaccharides to their cryoprotective performance. The basis of cryoprotection of FucoPol, pectin, and agar revealed a causal combination of (i) an emerging sol-gel transition ( p = 0.014) at near-hypothermia (4 °C), (ii) noncolligative attenuated supercooling of the kinetic freezing point of water ( p = 0.026) supporting ice growth anticipation, and (iii) increased conformational order ( p < 0.0001), where helix-/sheet-like features boost cryoprotection...
May 13, 2024: Biomacromolecules
https://read.qxmd.com/read/38739806/long-term-declines-in-chlorophyll-a-and-variable-phenology-revealed-by-a-60-year-estuarine-plankton-time-series
#2
JOURNAL ARTICLE
Patricia S Thibodeau, Gavino Puggioni, Jacob Strock, David G Borkman, Tatiana A Rynearson
Long-term ecological time series provide a unique perspective on the emergent properties of ecosystems. In aquatic systems, phytoplankton form the base of the food web and their biomass, measured as the concentration of the photosynthetic pigment chlorophyll a (chl a ), is an indicator of ecosystem quality. We analyzed temporal trends in chl a from the Long-Term Plankton Time Series in Narragansett Bay, Rhode Island, USA, a temperate estuary experiencing long-term warming and changing anthropogenic nutrient inputs...
May 21, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38739805/southern-ocean-drives-multidecadal-atmospheric-co-2-rise-during-heinrich-stadials
#3
JOURNAL ARTICLE
Kathleen A Wendt, Christoph Nehrbass-Ahles, Kyle Niezgoda, David Noone, Michael Kalk, Laurie Menviel, Julia Gottschalk, James W B Rae, Jochen Schmitt, Hubertus Fischer, Thomas F Stocker, Juan Muglia, David Ferreira, Shaun A Marcott, Edward Brook, Christo Buizert
The last glacial period was punctuated by cold intervals in the North Atlantic region that culminated in extensive iceberg discharge events. These cold intervals, known as Heinrich Stadials, are associated with abrupt climate shifts worldwide. Here, we present CO2 measurements from the West Antarctic Ice Sheet Divide ice core across Heinrich Stadials 2 to 5 at decadal-scale resolution. Our results reveal multi-decadal-scale jumps in atmospheric CO2 concentrations within each Heinrich Stadial. The largest magnitude of change (14...
May 21, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38739804/the-gpat4-6-8-clade-functions-in-arabidopsis-root-suberization-nonredundantly-with-the-gpat5-7-clade-required-for-suberin-lamellae
#4
JOURNAL ARTICLE
Kay Gully, Alice Berhin, Damien De Bellis, Cornelia Herrfurth, Ivo Feussner, Christiane Nawrath
Lipid polymers such as cutin and suberin strengthen the diffusion barrier properties of the cell wall in specific cell types and are essential for water relations, mineral nutrition, and stress protection in plants. Land plant-specific glycerol-3-phosphate acyltransferases (GPATs) of different clades are central players in cutin and suberin monomer biosynthesis. Here, we show that the GPAT4 / 6 / 8 clade in Arabidopsis thaliana , which is known to mediate cutin formation, is also required for developmentally regulated root suberization, in addition to the established roles of GPAT5/7 in suberization...
May 21, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38739795/the-physical-and-evolutionary-energy-landscapes-of-devolved-protein-sequences-corresponding-to-pseudogenes
#5
JOURNAL ARTICLE
Hana Jaafari, Carlos Bueno, Nicholas P Schafer, Jonathan Martin, Faruck Morcos, Peter G Wolynes
Protein evolution is guided by structural, functional, and dynamical constraints ensuring organismal viability. Pseudogenes are genomic sequences identified in many eukaryotes that lack translational activity due to sequence degradation and thus over time have undergone "devolution." Previously pseudogenized genes sometimes regain their protein-coding function, suggesting they may still encode robust folding energy landscapes despite multiple mutations. We study both the physical folding landscapes of protein sequences corresponding to human pseudogenes using the Associative Memory, Water Mediated, Structure and Energy Model, and the evolutionary energy landscapes obtained using direct coupling analysis (DCA) on their parent protein families...
May 21, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38739787/solvent-constraints-for-biopolymer-folding-and-evolution-in-extraterrestrial-environments
#6
JOURNAL ARTICLE
Ignacio E Sánchez, Ezequiel A Galpern, Diego U Ferreiro
We propose that spontaneous folding and molecular evolution of biopolymers are two universal aspects that must concur for life to happen. These aspects are fundamentally related to the chemical composition of biopolymers and crucially depend on the solvent in which they are embedded. We show that molecular information theory and energy landscape theory allow us to explore the limits that solvents impose on biopolymer existence. We consider 54 solvents, including water, alcohols, hydrocarbons, halogenated solvents, aromatic solvents, and low molecular weight substances made up of elements abundant in the universe, which may potentially take part in alternative biochemistries...
May 21, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38739784/seabirds-reveal-mercury-distribution-across-the-north-atlantic
#7
JOURNAL ARTICLE
Céline Albert, Børge Moe, Hallvard Strøm, David Grémillet, Maud Brault-Favrou, Arnaud Tarroux, Sébastien Descamps, Vegard Sandøy Bråthen, Benjamin Merkel, Jens Åström, Françoise Amélineau, Frédéric Angelier, Tycho Anker-Nilssen, Olivier Chastel, Signe Christensen-Dalsgaard, Johannis Danielsen, Kyle Elliott, Kjell Einar Erikstad, Alexey Ezhov, Per Fauchald, Geir W Gabrielsen, Maria Gavrilo, Sveinn Are Hanssen, Hálfdán H Helgason, Malin Kjellstadli Johansen, Yann Kolbeinsson, Yuri Krasnov, Magdalene Langset, Jérémy Lemaire, Svein-Håkon Lorentsen, Bergur Olsen, Allison Patterson, Christine Plumejeaud-Perreau, Tone K Reiertsen, Geir Helge Systad, Paul M Thompson, Thorkell Lindberg Thórarinsson, Paco Bustamante, Jérôme Fort
Mercury (Hg) is a heterogeneously distributed toxicant affecting wildlife and human health. Yet, the spatial distribution of Hg remains poorly documented, especially in food webs, even though this knowledge is essential to assess large-scale risk of toxicity for the biota and human populations. Here, we used seabirds to assess, at an unprecedented population and geographic magnitude and high resolution, the spatial distribution of Hg in North Atlantic marine food webs. To this end, we combined tracking data of 837 seabirds from seven different species and 27 breeding colonies located across the North Atlantic and Atlantic Arctic together with Hg analyses in feathers representing individual seabird contamination based on their winter distribution...
May 21, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38739642/artificial-neural-network-machine-learning-modelling-of-compressive-strength-of-recycled-coarse-aggregate-based-self-compacting-concrete
#8
JOURNAL ARTICLE
P Jagadesh, Afzal Hussain Khan, B Shanmuga Priya, A Asheeka, Zineb Zoubir, Hassan M Magbool, Shamshad Alam, Omer Y Bakather
This research study aims to understand the application of Artificial Neural Networks (ANNs) to forecast the Self-Compacting Recycled Coarse Aggregate Concrete (SCRCAC) compressive strength. From different literature, 602 available data sets from SCRCAC mix designs are collected, and the data are rearranged, reconstructed, trained and tested for the ANN model development. The models were established using seven input variables: the mass of cementitious content, water, natural coarse aggregate content, natural fine aggregate content, recycled coarse aggregate content, chemical admixture and mineral admixture used in the SCRCAC mix designs...
2024: PloS One
https://read.qxmd.com/read/38739619/correction-ecological-niche-modelling-of-two-water-dependant-birds-informs-the-conservation-needs-of-riverine-ecosystems-outside-protected-area-network-in-the-eastern-himalaya-india
#9
Roshan Tamang, Vallanattu James Jins, Sailendra Dewan, Shivaji Chaudhry, Seema Rawat, Bhoj Kumar Acharya
[This corrects the article DOI: 10.1371/journal.pone.0294056.].
2024: PloS One
https://read.qxmd.com/read/38739345/gafchromic-ebt3-film-provides-equivalent-dosimetric-performance-to-ebt-xd-film-for-stereotactic-radiosurgery-dosimetry
#10
JOURNAL ARTICLE
Lloyd Smyth, Andrew Alves, Katherine Collins, Sabeena Beveridge
The accurate assessment of film results is highly dependent on the methodology and techniques used to process film. This study aims to compare the performance of EBT3 and EBT-XD film for SRS dosimetry using two different film processing methods. Experiments were performed in a solid water slab and an anthropomorphic head phantom. For each experiment, the net optical density of the film was calculated using two different methods; taking the background (initial) optical density from 1) an unirradiated film from the same film lot as the irradiated film (stock to stock (S-S) method), and 2) a scan of the same piece of film taken prior to irradiation (film to film (F-F) method)...
May 13, 2024: Physical and engineering sciences in medicine
https://read.qxmd.com/read/38739339/in-depth-characterization-of-particulate-matter-in-a-highly-polluted-urban-environment-at-the-foothills-of-himalaya-karakorum-region
#11
JOURNAL ARTICLE
Bahadar Zeb, Khan Alam, Zhongwei Huang, Fatma Öztürk, Peng Wang, Lyudmila Mihaylova, Muhammad Fahim Khokhar, Said Munir
In recent years, the rising levels of atmospheric particulate matter (PM) have an impact on the earth's system, leading to undesirable consequences on various aspects like human health, visibility, and climate. The present work is carried out over an insufficiently studied but polluted urban area of Peshawar, which lies at the foothills of the famous Himalaya and Karakorum area, Northern Pakistan. The particulate matter with an aerodynamic diameter of less than 10 µm, i.e., PM10 are collected and analyzed for mineralogical, morphological, and chemical properties...
May 13, 2024: Environmental Science and Pollution Research International
https://read.qxmd.com/read/38739337/water-quality-characteristics-and-ecological-risk-evaluation-of-a-landscaped-river-replenished-by-three-reclaimed-water-sources-in-qingdao-china
#12
JOURNAL ARTICLE
Yanan Cai, Yunze Bi, Bowen Tian, Lihua Cheng, Shuhui Zhou, Quanyong Qi
The water crisis may be solved by utilizing reclaimed water. Three reclaimed water sources have restored the lower sections of the Licun River, forming a landscaped river. In this paper, the river's water quality was monitored for a year, and the ecological concerns were analyzed using luminescent bacteria, chlorella, and zebrafish. The results indicated that although basic water quality indicators like COD and ammonia fluctuated along the river, the classification of water quality was primarily affected by factors such as flow rate and water depth...
May 13, 2024: Environmental Science and Pollution Research International
https://read.qxmd.com/read/38739202/morpho-physiological-studies-of-sandalwood-host-interaction-under-individual-and-interactive-water-and-salt-stress
#13
JOURNAL ARTICLE
Aarju Sharma, Ashwani Kumar, Sulekha Chahal, Kamlesh Verma, Kajal Chauhan, Raj Kumar, Anita Mann
To find out the possibilities of growing white sandalwood in sub-tropical regions of India where farmers facing the problem of water deficit and salinity stress, a RBD experiment was conducted. Sandalwood grown alone and with five selected hosts (Alternanthera sp., Neem, Shisham, Dek and Agarwood) on the basis of prior study under water deficit, salinity stress and combined water deficit and salinity stress. Sandalwood plants were harvested after 180 days of imposing stress treatments. Morphological traits (plant height, collar diameter, shoot fresh and dry biomass) showed significant reduction under water deficit and salinity stress, which were further aggravated under combined water deficit and salinity stress...
May 13, 2024: Biologia futura
https://read.qxmd.com/read/38738893/microhardness-measurements-on-tooth-and-alveolar-bone-in-rodent-oral-disease-models
#14
JOURNAL ARTICLE
Motoki Okamoto, Shohei Yamashita, Takumi Memida, Melanie Mendonca, Susanne Brueckner, Shin Nakamura, Satoru Shindo, Ria Achong-Bowe, Jeffrey Thompson, Xiaozhe Han, Toshihisa Kawai, Maiko Suzuki
The mechanical property, microhardness, is evaluated in dental enamel, dentin, and bone in oral disease models, including dental fluorosis and periodontitis. Micro-CT (µCT) provides 3D imaging information (volume and mineral density) and scanning electron microscopy (SEM) produces microstructure images (enamel prism and bone lacuna-canalicular). Complementarily to structural analysis by µCT and SEM, microhardness is one of the informative parameters to evaluate how structural changes alter mechanical properties...
April 26, 2024: Journal of Visualized Experiments: JoVE
https://read.qxmd.com/read/38738519/the-mitigative-role-of-novel-aflatoxin-degrading-enzymes-in-diverse-broiler-performance-indicators-and-gut-microbiota-following-the-consumption-of-diets-contaminated-with-aflatoxins
#15
JOURNAL ARTICLE
Ru Jia, Senmiao Tian, Zhaofeng Yang, Simeng Lu, Lan Wang, Guohua Zhang
BACKGROUND: This study aims to explore both the toxic effects of aflatoxins (AFs) and the protective effects of degrading enzymes (DE) on broilers exposed to AFs. RESULTS: The findings reveal that a diet contaminated with 69.15 μg kg-1 of aflatoxin B1 had significant adverse effects on broilers. Specifically, it led to a reduction in average daily gain, dressed yield percentage, half-eviscerated yield with giblet yield percentage, eviscerated yield percentage, as well as serum superoxide dismutase (SOD), glutathione peroxidase activity and liver SOD activity (P < 0...
May 13, 2024: Journal of the Science of Food and Agriculture
https://read.qxmd.com/read/38738265/honey-induces-changes-in-the-molecular-structure-and-microstructure-of-gluten-in-wheat-rye-sourdoughs
#16
JOURNAL ARTICLE
Julia Nutter, Amelia Ivone Saiz
Chemical oxidizers and redox enzymes have traditionally been used to enhance the quality of baked goods. However, consumers now seek natural and clean-label ingredients, avoiding those with chemical-sounding names. Honey, a natural source of glucose oxidase (GOX), represents a promising alternative to purified enzymes for baking purposes. This study aimed to evaluate the effect of honey on the molecular structure and microstructure of gluten proteins in sourdough fermented by different lactic acid bacteria (LAB) strains...
May 13, 2024: Food Science and Technology International
https://read.qxmd.com/read/38737510/piloting-a-brief-assessment-to-capture-consumption-of-whole-plant-food-and-water-version-1-0-of-the-american-college-of-lifestyle-medicine-diet-screener-aclm-diet-screener
#17
JOURNAL ARTICLE
Micaela C Karlsen, Kara L Staffier, Kathryn J Pollard, Kelly C Cara, Sarah M Hulit, Erin K Campbell, Susan M Friedman
BACKGROUND: Despite the availability of various dietary assessment tools, there is a need for a tool aligned with the needs of lifestyle medicine (LM) physicians. Such a tool would be brief, aimed at use in a clinical setting, and focused on a "food as medicine" approach consistent with recommendations for a diet based predominately on whole plant foods. The objective of this study is to describe the development and initial pilot testing of a brief, dietary screener to assess the proportion of whole, unrefined plant foods and water relative to total food and beverage intake...
2024: Frontiers in Nutrition
https://read.qxmd.com/read/38737244/dihydroquercetin-regulates-hif-1%C3%AE-akt-nr2b-signalling-to-improve-impaired-brain-function-in-rats-with-metabolic-syndrome
#18
JOURNAL ARTICLE
Yang Fu, PeiPei Yuan, Mengnan Zeng, Qi Zhang, Ying Hou, Liyuan Gao, Yaxin Wei, Yajuan Zheng, Weisheng Feng, Xiaoke Zheng
Dihydroquercetin (DHQ) is commonly used as a dietary additive, but its activity in improving brain injury with metabolic syndrome (MS) remains known. In present study, the MS rat model was induced using 10 % fructose water. The apoptosis rate of primary brain cells was detected. The HIF-1α/AKT/NR2B signalling pathway, levels of KEAP1/NRF2, HO-1 and NQO-1 were detected. In vitro experiments were performed using H2 O2 -stimulated PC-12 cells. The effect of DHQ on rates of cell survival and apoptosis were detected...
May 15, 2024: Heliyon
https://read.qxmd.com/read/38736441/non-destructive-assessment-of-cannabis-quality-during-drying-process-using-hyperspectral-imaging-and-machine-learning
#19
JOURNAL ARTICLE
Hyo In Yoon, Su Hyeon Lee, Dahye Ryu, Hyelim Choi, Soo Hyun Park, Je Hyeong Jung, Ho-Youn Kim, Jung-Seok Yang
Cannabis sativa L. is an industrially valuable plant known for its cannabinoids, such as cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC), renowned for its therapeutic and psychoactive properties. Despite its significance, the cannabis industry has encountered difficulties in guaranteeing consistent product quality throughout the drying process. Hyperspectral imaging (HSI), combined with advanced machine learning technology, has been used to predict phytochemicals that presents a promising solution for maintaining cannabis quality control...
2024: Frontiers in Plant Science
https://read.qxmd.com/read/38736336/selected-ice-nanoparticle-accelerator-hypervelocity-impact-mass-spectrometer-selina-hims-features-and-impacts-of-charged-particles
#20
JOURNAL ARTICLE
Anatolii Spesyvyi, Ján Žabka, Miroslav Polášek, Michaela Malečková, Nozair Khawaja, Jürgen Schmidt, Sascha Kempf, Frank Postberg, Ales Charvat, Bernd Abel
The selected ice nanoparticle accelerator, SELINA, was used to prepare beams of single ice particles with positive or negative charge. Positively charged particles were prepared from deionized water and 0.05-0.2 molar solutions of sodium chloride in water, and negatively charged ice particles were generated from water without salt. Depending on the electrospray source configuration, the measured particles vary from 50 to 1000 nm in diameter. The kinetic energy per charge for all particles was set to 200 eV by the collisional equilibration in quadrupoles, which resulted in primary velocities up to 600 m/s for the lowest m/z particles...
June 9, 2024: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
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