Jiyoon Bu, Woo-Jin Jeong, Roya Jafari, Luke J Kubiatowicz, Ashita Nair, Michael J Poellmann, Rachel S Hong, Elizabeth W Liu, Randall H Owen, Piper A Rawding, Caroline M Hopkins, DaWon Kim, Daniel J George, Andrew J Armstrong, Petr Král, Andrew Z Wang, Justine Bruce, Tian Zhang, Randall J Kimple, Seungpyo Hong
Despite its high potential, PD-L1 expressed by tumors has not been successfully utilized as a biomarker for estimating treatment responses to immunotherapy. Circulating tumor cells (CTCs) and tumor-derived exosomes that express PD-L1 can potentially be used as biomarkers; however, currently available assays lack clinically significant sensitivity and specificity. Here, a novel peptide-based capture surface is developed to effectively isolate PD-L1-expressing CTCs and exosomes from human blood. For the effective targeting of PD-L1, this study integrates peptide engineering strategies to enhance the binding strength and specificity of a β-hairpin peptide derived from PD-1 (pPD-1)...
October 1, 2022: Biosensors & Bioelectronics