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MicroRNAs and early detection

Zhen He, Jian Jun Yang, Rong Zhang, Hua Ting Li, Liang Wu, Feng Jiang, Wei Ping Jia, Cheng Hu
AIM: The early screening of non-alcoholic fatty liver disease (NAFLD) is of great significance for early detection and intervention in NAFLD. MicroRNAs (miRNAs) are important regulators of metabolic disease, including NAFLD. The aim of this study was to examine the association of serum miR-29a-c with NAFLD in a Chinese population. METHODS: 276 individuals were enrolled in this study and divided into non-diabetic and diabetic groups. Real-time PCR was performed to quantify the serum miR-29a-c expression levels...
February 12, 2019: Journal of Digestive Diseases
Dongli Pan, Gang Li, Jenna Morris-Love, Shuyuan Qi, Lei Feng, Max E Mertens, Igor Jurak, David M Knipe, Donald M Coen
Herpes simplex virus 1 (HSV-1) switches between two infection programs, productive ("lytic") and latent infection. Some HSV-1 microRNAs (miRNAs) have been hypothesized to help control this switch, and yet little is known about regulation of their expression. Using Northern blot analyses, we found that, despite inherent differences in biogenesis efficiency among six HSV-1 miRNAs, all six exhibited high pre-miRNA/miRNA ratios during lytic infection of different cell lines and, when detectable, in acutely infected mouse trigeminal ganglia...
February 12, 2019: MBio
Yuanfeng Pang, Chaoguang Wang, LuChun Lu, Chongwen Wang, Zhiwei Sun, Rui Xiao
MicroRNAs have been proved to be the biomarker for early detection of pancreatic cancer and the precisely quantitation of the MicroRNA-10b in the blood samples even can distinguish pancreatic cancer from chronic pancreatitis (CP) and normal controls (NC). In this study, we developed a DSN-assisted dual-SERS biosensor for microRNA-10b in exosome and residual plasma of blood samples detection based on the Fe3 O4 @Ag-DNA-Au@Ag@DTNB (SERS tag) conjugates. In presence of target microRNA, it can hybridized with the complementary DNA probes...
January 25, 2019: Biosensors & Bioelectronics
Daniel Gilbert Weber, Alexander Brik, Swaantje Casjens, Katarzyna Burek, Martin Lehnert, Beate Pesch, Dirk Taeger, Thomas Brüning, Georg Johnen
OBJECTIVE: Malignant mesothelioma is an aggressive cancer of the serous membranes. For the detection of the tumor at early stages non- or minimally-invasive biomarkers are needed. The circulating biomarkers miR-132-3p, miR-126-3p, and miR-103a-3p were analyzed in a nested case-control study using plasma samples from 17 prediagnostic mesothelioma cases and 34 matched asbestos-exposed controls without a malignant disease. RESULTS: Using prediagnostic plasma samples collected in median 8...
February 11, 2019: BMC Research Notes
Yuan Hu, An-Yue Wu, Cong Xu, Ke-Qi Song, Wen-Jing Wang, Xia Yin, Wen Di, Zu-Bei Hong, Li-Hua Qiu
Endometrial cancer represents the leading frequency in gynecological malignancy in developed countries. Even with early detection, metastasis and recurrence remain the main reasons for a high death rate. MicroRNA-449a (miR-449a) has been reported to function as a tumor suppressor, yet the role of miR-449a in endometrial cancer metastasis has not been investigated. The present study identified that miR-449a was downregulated in advanced endometrial cancer. Overexpression of miR-449a decreased the migration and invasion of KLE and AN3CA endometrial cancer cells...
2019: Journal of Cancer
Liangxi Yuan, Jian Dong, Guanglang Zhu, Junmin Bao, Qingsheng Lu, Jian Zhou, Zaiping Jing
In-stent restenosis (ISR) is still a major cause of failure of endovascular stenting treatment in patients with lower extremity arterial occlusive disease (LEAOD). Sensitive and reliable biomarkers for early diagnosis to predict ISR should be considered. This study was conducted to explore the diagnostic value of microRNA in predicting ISR in patients with LEAOD after endovascular stenting treatment. From March 2014 to July 2016, 208 patients (170 males and 38 females) with LEAOD undergoing interventional treatment were enrolled in this research...
February 4, 2019: Scientific Reports
Mohamad Mahani, Zhahra Mousapour, Faten Divsar, Alireza Nomani, Huangxian Ju
A carbon quantum dot (CQD) labeled molecular beacon was synthesized and applied to the detection of microRNA-21. The CQDs possess low cytotoxicity, excellent water solubility, and photostability. The CQDs were characterized by transmission electron microscopy, dynamic light scattering, Fourier-transform infrared spectroscopy, and fluorescence spectroscopy. The molecular beacon (MB) was labeled with the CQDs at the 5' end, and with Black Hole Quencher 1 (BHQ1) at the 3' end. The two labels act as the donor and acceptor parts of a FRET system, respectively...
February 1, 2019: Mikrochimica Acta
Zebo Huang, Wenjiao Chen, Yiping Du, Qin Guo, Yong Mao, Xin Zhou, Dong Hua
BACKGROUND: Cancer is a serious public health problem worldwide, and difficulty in early diagnosis has been the chief obstacle to improve the prognosis of patients. Recently, microRNAs (miRNAs) were widely studied to be potential biomarkers for cancer detection. miR-16 is a prevalent but sophisticated one. In the current study, we aimed to assess the diagnostic value of serum miR-16 for cancer detection. METHODS: A total of 1458 cancer patients, containing ten types of cancers, and 1457 non-cancer controls were recruited in this study...
January 31, 2019: Journal of Cancer Research and Clinical Oncology
Marta Herreros-Villanueva, Saray Duran-Sanchon, Ana Carmen Martín, Rosa Pérez-Palacios, Elena Vila-Navarro, María Marcuello, Mireia Diaz-Centeno, Joaquín Cubiella, Maria Soledad Diez, Luis Bujanda, Angel Lanas, Rodrigo Jover, Vicent Hernández, Enrique Quintero, Juan José Lozano, Marta García-Cougil, Ibon Martínez-Arranz, Antoni Castells, Meritxell Gironella, Rocio Arroyo
OBJECTIVES: Specific microRNA (miRNA) signatures in biological fluids can facilitate earlier detection of the tumors being then minimally invasive diagnostic biomarkers. Circulating miRNAs have also emerged as promising diagnostic biomarkers for colorectal cancer (CRC) screening. In this study, we investigated the performance of a specific signature of miRNA in plasma samples to design a robust predictive model that can distinguish healthy individuals from those with CRC or advanced adenomas (AA) diseases...
January 2019: Clinical and Translational Gastroenterology
Tinglan Ouyang, Zhiyu Liu, Zhiyi Han, Qinyu Ge
MicroRNAs (miRNAs) are a series of promising molecules that could regulate gene expression, while its expression level and type are strictly related to the early diagnosis, targeted therapy and prognostic of diseases. Improved detection accuracy of miRNAs would lead to unprecedented progress in early treatment. Numerous methods to improve sensitivity have been proposed and confirmed valuable in miRNAs detection recently. However, the research which especially targets at improving detection specificity remains rare...
January 31, 2019: Analytical Chemistry
Jiafang Piao, Qian Zhao, Dianming Zhou, Weipan Peng, Weichen Gao, Minghui Chen, Guiming Shu, Xiaoqun Gong, Jin Chang
MicroRNAs (miRNAs) were reported to be potential tumor markers for early diagnosis of cancer. Due to its short sequence, low expression level and high susceptibility to degradation, the stable and sensitive detection method of miRNAs is arduous to establish. In this work, we designed a metal chelator (ethylenediamine tetraacetic acid disodium salt, EDTA•2Na) labeled oligonucleotides as the plasmonic signal supraregulator probe to control the generation of gold nanoparticles (AuNPs). Based on another complementary oligonucleotides of target miRNA labeling SiO2 microparticles (SiO2 MPs) as the detecting platform, EDTA•2Na labeled oligonucleotide probes were immobilized on the SiO2 platform through the sandwich structure in the presence of target miRNA...
April 4, 2019: Analytica Chimica Acta
Takumi Kagawa, Tomáš Zárybnický, Takanao Omi, Yuji Shirai, Shinya Toyokuni, Shingo Oda, Tsuyoshi Yokoi
Drug-induced acute kidney injury (AKI) is a frequent cause of adverse drug reaction. Serum creatinine (CRE) and blood urea nitrogen (BUN) are widely used as standard biomarkers for kidney injury; however, the sensitivity and specificity are considered to be low. In recent years, circulating microRNA (miRNAs) have been attracting considerable attention as novel biomarkers for organ injury, but there are currently no established miRNA biomarkers for drug-induced AKI. The present study aimed to identify plasma miRNAs that may enable early and specific detection of drug-induced tubular and glomerular injury through next-generation sequencing analysis...
January 22, 2019: Toxicology
Yonghong Zhang, Gang Yang, Yicheng Luo
Glioma is a type of malignant tumor accounting for 80% of all brain cancer morbidity. The long non-coding RNA (lncRNA) PVT1 has been demonstrated to be an oncogenic lncRNA in other types of cancer. However, the role of PVT1 in glioma is still unknown. The aim of the present study was to investigate the role of PVT1 in glioma, and its potential association with microRNA (miR)-200a. miR-200a mimics and small interfering (si)RNA transfection were utilized to construct miR-200a overexpression and knockdown models to investigate the effect of miR-200a on glioma cells...
February 2019: Experimental and Therapeutic Medicine
Hong Yu, Jianying Sun, Shaochang Jiang, Ying Xu
microRNA (miRNA) expression profiles of gastric cancer (GC) and adjacent healthy gastric mucosa tissue were used to search for differentially expressed miRNAs, identifying downregulated miRNA-490-3p in GC. The present study aimed to investigate the cellular function of miRNA-490-3p and its underlying mechanism in the occurrence and progression of GC. Reverse transcription-quantitative polymerase chain reaction was used to measure miRNA-490-3p expression levels in GC tissue and adjacent healthy tissue samples...
February 2019: Experimental and Therapeutic Medicine
Yusuke Okuno, Takayuki Murata, Yoshitaka Sato, Hideki Muramatsu, Yoshinori Ito, Takahiro Watanabe, Tatsuya Okuno, Norihiro Murakami, Kenichi Yoshida, Akihisa Sawada, Masami Inoue, Keisei Kawa, Masao Seto, Koichi Ohshima, Yuichi Shiraishi, Kenichi Chiba, Hiroko Tanaka, Satoru Miyano, Yohei Narita, Masahiro Yoshida, Fumi Goshima, Jun-Ichi Kawada, Tetsuya Nishida, Hitoshi Kiyoi, Seiichi Kato, Shigeo Nakamura, Satoko Morishima, Tetsushi Yoshikawa, Shigeyoshi Fujiwara, Norio Shimizu, Yasushi Isobe, Masaaki Noguchi, Atsushi Kikuta, Keiji Iwatsuki, Yoshiyuki Takahashi, Seiji Kojima, Seishi Ogawa, Hiroshi Kimura
Epstein-Barr virus (EBV) infection is highly prevalent in humans and is implicated in various diseases, including cancer1,2 . Chronic active EBV infection (CAEBV) is an intractable disease classified as a lymphoproliferative disorder in the 2016 World Health Organization lymphoma classification1,2 . CAEBV is characterized by EBV-infected T/natural killer (NK) cells and recurrent/persistent infectious mononucleosis-like symptoms3 . Here, we show that CAEBV originates from an EBV-infected lymphoid progenitor that acquires DDX3X and other mutations, causing clonal evolution comprising multiple cell lineages...
January 21, 2019: Nature Microbiology
Xiang-Ming Hu, Rong-Tian Li, Miao-Miao Zhang, Ke-Yang Wu, Huan-Huan Li, Nai-Han Huang, Bin Sun, Jin-Xiang Chen
A phenanthroline (phen) tethered berberine dimer 1 is synthesized and further conjugated with carboxyfluorescein (FAM)-labeled single-stranded probe DNA (P-DNA) to give P-DNA@1. The mutual interaction of these two components triggers the fluorescence quenching of FAM, and the non-emissive P-DNA@1, in turn, functions as a sensor to detect cancer-associated microRNA-185 (miRNA-185), characterized by the FAM fluorescence recovery. The results show that P-DNA@1 is capable of detecting miRNA-185 in 2 min with the detection limit of 0...
March 21, 2019: Analytica Chimica Acta
Md Rezanur Rahman, Tania Islam, Esra Gov, Beste Turanli, Gizem Gulfidan, Md Shahjaman, Nilufa Akhter Banu, Md Nurul Haque Mollah, Kazim Yalcin Arga, Mohammad Ali Moni
Colorectal cancer (CRC) is the second most common cause of cancer-related death in the world, but early diagnosis ameliorates the survival of CRC. This report aimed to identify molecular biomarker signatures in CRC. We analyzed two microarray datasets (GSE35279 and GSE21815) from the Gene Expression Omnibus (GEO) to identify mutual differentially expressed genes (DEGs). We integrated DEGs with protein⁻protein interaction and transcriptional/post-transcriptional regulatory networks to identify reporter signaling and regulatory molecules; utilized functional overrepresentation and pathway enrichment analyses to elucidate their roles in biological processes and molecular pathways; performed survival analyses to evaluate their prognostic performance; and applied drug repositioning analyses through Connectivity Map (CMap) and geneXpharma tools to hypothesize possible drug candidates targeting reporter molecules...
January 17, 2019: Medicina
Xiu Wei Zhang, Lei Wang, Hong Ding
Peritoneal fibrosis (PF) represents a well-recognized complication associated with continuous ambulatory peritoneal dialysis therapy, characterized by a reversible epithelial-to-mesenchymal transition (EMT) at the early stage. The aim of the current study was to investigate the effects linked with the long noncoding RNA (lncRNA) AK089579 on the EMT of peritoneal mesothelial cells (PMCs) as well as the associated regulatory mechanisms of AK089579 downstream of tyrosine kinase 2 (DOK2) and microRNA-296-3p (miR-296-3p)...
January 17, 2019: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Helal F Hetta, Asmaa M Zahran, Engy A Shafik, Reham I El-Mahdy, Nahed A Mohamed, Emad Eldin Nabil, Hend M Esmaeel, Ola A Alkady, Azza Elkady, Dina A Mohareb, Amal Hosni, Mohammed Mahmoud Mostafa, Abeer Elkady
BACKGROUND AND AIM: Lung cancer (LC) is a major cancer killer worldwide, and 5-yr survival is extremely poor (≤15%), accentuating the need for more effective diagnostic and therapeutic strategies. Studies have shown cell-free microRNAs (miRNAs) circulating in the serum and plasma with specific expression in cancer, indicating the potential of using miRNAs as biomarkers for cancer diagnosis and therapy. This study aimed to identify differentially-expressed two miRNAs in the plasma of non-small cell lung cancer (NSCLC) patients that might be a clinically useful tool for lung cancer early detection...
January 15, 2019: MicroRNA
Chunrong Zhao, Ying Miao, Zhen Cao, Jian Shi, Jianmei Li, Fei Kang, Ce Dou, Zhao Xie, Qiang Xiang, Shiwu Dong
OBJECTIVE: Chondrocyte hypertrophy, a terminal stage of chondrocyte differentiation, is essential to the endochondral bone formation and is one of the major pathological factors in osteoarthritis. This study investigated the role of microRNA-29b (miR-29b), which is involved in chondrogenesis, in the regulation of hypertrophy in chondrocytes. METHODS: miR-29b expression was assessed during murine mesenchymal stem cells (mMSCs) chondrogenesis. To detect whether miR-29b affects chondrocyte hypertrophy, the mMSCs induced toward chondrogenesis were transfected with miR-29b or its antisense inhibitor (antagomiR-29b)...
January 16, 2019: Journal of Cellular Biochemistry
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