Aimilia Nousi, Gustavo Avelar Molina, Corinna Schiano-di-Cola, Trine Holst Sørensen, Kim Borch, Jonas N Pedersen, Peter Westh, Rodolphe Marie
Enzymatic degradation of cellulosic biomass is a well-established route for the sustainable production of biofuels, chemicals, and materials. A strategy employed by nature and industry to achieve an efficient degradation of cellulose is that cellobiohydrolases (or exocellulases), such as Cel7A, work synergistically with endoglucanases, such as Cel7B, to achieve the complete degradation of cellulose. However, a complete mechanistic understanding of this exo-endo synergy is still lacking. Here, we used single-molecule fluorescence microscopy to quantify the binding kinetics of Cel7A on cellulose when it is acting alone on the cellulose fibrils and in the presence of its synergy partner, the endoglucanase Cel7B...
January 16, 2024: Journal of Physical Chemistry. B