Konstantin V Lavrov, Anna O Shemyakina, Elena G Grechishnikova, Tatyana V Gerasimova, Tatyana I Kalinina, Andrey D Novikov, Tatyana E Leonova, Ludmila E Ryabchenko, Telman A Bayburdov, Alexander S Yanenko
Rhodococcus strains were designed as model biocatalysts (BCs) for the production of acrylic acid and mixtures of acrylic monomers consisting of acrylamide, acrylic acid, and N-alkylacrylamide (N-isopropylacrylamide). To obtain BC strains, we used, among other approaches, adaptive laboratory evolution (ALE), based on the use of the metabolic pathway of amide utilization. Whole genome sequencing of the strains obtained after ALE, as well as subsequent targeted gene disruption, identified candidate genes for three new amidases that are promising for the development of BCs for the production of acrylic acid from acrylamide...
June 2024: Metabolic Engineering Communications